Genotoxicity assessment of membrane concentrates of landfill leachate treated with Fenton reagent and UV-Fenton reagent using human hepatoma cell line

Journal of Hazardous Materials
Guifang WangQiming Jimmy Yu

Abstract

Membrane concentrates of landfill leachates contain organic and inorganic contaminants that could be highly toxic and carcinogenic. In this paper, the genotoxicity of membrane concentrates before and after Fenton and UV-Fenton reagent was assessed. The cytotoxicity and genotoxicity was determined by using the methods of methyltetrazolium (MTT), cytokinesis-block micronucleus (CBMN) and comet assay in human hepatoma cells. MTT assay showed a cytotoxicity of 75% after 24h of exposure to the highest tested concentration of untreated concentrates, and no cytotoxocity for UV-Fenton and Fenton treated concentrates. Both CBMN and comet assays showed increased levels of genotoxicity in cells exposed to untreated concentrates, compared to those occurred in cells exposed to UV-Fenton and Fenton reagent treated concentrates. There was no significant difference between negative control and UV-Fenton treated concentrates for micronucleus and comet assay parameters. UV-Fenton and Fenton treatment, especially the former, were effective methods for degradation of bisphenol A and nonylphenol in concentrates. These findings showed UV-Fenton and Fenton reaction were effective methods for treatment of such complex concentrates, UV-Fenton reagent p...Continue Reading

References

Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Sep 12, 2003·Environmental Science & Technology·Bart Van der BruggenCarlo Vandecasteele
Nov 26, 2003·Chemosphere·Antonio LopezAppio Claudio Di Pinto
Jan 30, 2004·Environment International·Junko Sajiki, Jun Yonekubo
Jul 14, 2005·Journal of Hazardous Materials·Hui ZhangChin-Pao Huang
Aug 9, 2005·The Science of the Total Environment·Saurabh ChandraS K Gupta
Sep 15, 2005·Journal of Hazardous Materials·Hung-Yee ShuWen-Pin Hsieh
Jan 13, 2006·Ecotoxicology and Environmental Safety·Nan SangXiaoyun Xin
Apr 14, 2007·International Journal of Environmental Research and Public Health·Onyinye NwagbaraRichard D Gragg
Jun 5, 2007·Nature Protocols·Michael Fenech
Sep 14, 2007·Chemico-biological Interactions·Neife Aparecida Guinaim dos SantosAntonio Cardozo dos Santos
Apr 19, 2008·Environmental Toxicology and Chemistry·Terence TaloreteHiroko Isoda
Jun 17, 2008·Cell Biology and Toxicology·S M Piperakis
Jan 2, 2009·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·David John Lawrence ThomasSteve Farrow
May 20, 2009·Water Research·Paul WesterhoffJohn Crittenden
Feb 13, 2010·Journal of Environmental Management·Latesh B Chaudhari, Z V P Murthy
Jun 18, 2010·Journal of Hazardous Materials·Paolo S CalabròCarlo Meoni
Apr 26, 2011·Ecotoxicology and Environmental Safety·Goran GajskiVera Garaj-Vrhovac
Dec 20, 2011·Ecotoxicology and Environmental Safety·Goran GajskiVera Garaj-Vrhovac
Jun 6, 2012·TheScientificWorldJournal·Kamila WidziewiczPaweł Madej
Aug 23, 2012·Ecotoxicology and Environmental Safety·Karine BertoldiIonara Rodrigues Siqueira
Feb 8, 2014·Scientific Reports·Peng LiuWenfang Zhang
Mar 22, 2014·Environmental Technology·Chuanrui JiangQiongfang Zhuo
Mar 29, 2014·Environmental Science and Pollution Research International·Pooja GhoshIndu Shekhar Thakur
Aug 13, 2014·Bioresource Technology·Pooja GhoshIndu Shekhar Thakur

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Citations

Jul 6, 2016·Archives of Toxicology·M GleiW Schlörmann
Apr 1, 2017·Ecotoxicology and Environmental Safety·Pooja GhoshAnubha Kaushik
Dec 7, 2017·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Mianwei HongLingfei Zhu
Oct 28, 2019·Environmental Science and Pollution Research International·Xu RenDan Liu

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