Genotoxicity assessment of reactive and disperse textile dyes using human dermal equivalent (3D cell culture system)

Journal of Toxicology and Environmental Health. Part a
Daniela Morais LemeDanielle Palma de Oliveira

Abstract

Thousands of dyes are marketed daily for different purposes, including textile dyeing. However, there are several studies reporting attributing to dyes deleterious human effects such as DNA damage. Humans may be exposed to toxic dyes through either ingestion of contaminated waters or dermal contact with colored garments. With respect to dermal exposure, human skin equivalents are promising tools to assess in vitro genotoxicity of dermally applied chemicals using a three-dimensional (3D) model to mimic tissue behavior. This study investigated the sensitivity of an in-house human dermal equivalent (DE) for detecting genotoxicity of textile dyes. Two azo (reactive green 19 [RG19] and disperse red 1[DR1]) dyes and one anthraquinone (reactive blue 2 [RB2]) dye were analyzed. RG19 was genotoxic for DE in a dose-responsive manner, whereas RB2 and DR1 were nongenotoxic under the conditions tested. These findings are not in agreement with previous genotoxicological assessment of these dyes carried out using two-dimensional (2D) cell cultures, which showed that DR1 was genotoxic in human hepatoma cells (HepG2) and RG19 was nongenotoxic for normal human dermal fibroblasts (NHDF). These discrepant results probably may be due to differences...Continue Reading

References

Jun 1, 1978·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·L C JunqueiraR Brentani
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E BellC Merrill
Dec 1, 1979·Mutation Research·S Venturini, M Tamaro
Sep 1, 1992·Mutation Research·K T Chung, C E Cerniglia
Jul 15, 1991·Experientia·T D NguyenF X Maquart
Jan 1, 1988·Archives of Dermatological Research·L W Adams, G C Priestley
May 1, 1997·Archives of Dermatological Research·M PonecA M Mommaas
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Jun 16, 2004·Environment International·Esther ForgacsGyula Oros
Jul 1, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Abdoelwaheb El Ghalbzouri, Maria Ponec
May 25, 2005·Chemosphere·Gisela de Aragão UmbuzeiroLarry D Claxton
Jun 7, 2005·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·E BehraveshS Gonda
Jul 7, 2005·Tissue Engineering·Annie F BlackOdile Damour
Dec 21, 2005·Chemical Society Reviews·Desmond J Tobin
Jun 20, 2006·Mutation Research·Rodger D CurrenMarilyn J Aardema
Oct 10, 2006·Mutation Research·Rodrigo Otávio Alves de LimaGisela de Aragão Umbuzeiro
Oct 31, 2006·Mutation Research·Danielle P OliveiraGisela A Umbuzeiro
Oct 31, 2006·Archives of Dermatological Research·Yves Poumay, Alain Coquette
Jun 2, 2007·Menopause International·J Calleja-AgiusM P Brincat
Aug 31, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M S TsuboyL R Ribeiro
Nov 28, 2007·Cell Biology and Toxicology·T S KumaravelAwadhesh N Jha
Apr 25, 2008·Current Protocols in Immunology·W Strober
Nov 14, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Craig K Svensson
Dec 24, 2008·Genes & Nutrition·Giovanna MazzoleniN Steimberg
Apr 29, 2009·Experimental Dermatology·Martina MeinkeJürgen Lademann
May 16, 2009·Mutation Research·Farah Maria Drumond ChequerDanielle Palma de Oliveira
Dec 23, 2009·Toxicology and Applied Pharmacology·Mai A Ngo, Howard I Maibach
Oct 30, 2010·Pigment Cell & Melanoma Research·Carla A BrohemSilvya S Maria-Engler
Nov 12, 2010·Tissue Engineering. Part a·Jessica JeanRoxane Pouliot
Jan 5, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Thomas Hartung
Jan 19, 2011·Advanced Drug Delivery Reviews·Florian GroeberKatja Schenke-Layland
Feb 4, 2011·Journal of Bioscience and Bioengineering·Katsumi EbisawaHideaki Kagami

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Citations

May 2, 2018·Journal of Toxicology and Environmental Health. Part a·Daniela Morais LemeDanielle Palma de Oliveira
Nov 16, 2018·Chemosphere·Crislaine Maria Carvalho da Cruz BrambillaJuliana da Silva
Jul 26, 2019·Chemosphere·Flavia R AbeJoão L T Pestana

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