Genotoxicity evaluation of asymmetric lipid polymer hybrid nanoparticles of doxycycline hydrochloride following intravenous administration

Drug Delivery and Translational Research
Maheshkumar P SoniGeeta R Vanage

Abstract

Nanoparticles, being small (<1,000 nm) in size, provide high surface area-to-volume ratio as compared with the bulk materials which increase the concern about their potential toxicities. The present investigation was undertaken to evaluate the genotoxic potential of asymmetric lipid polymer hybrid nanoparticles of doxycycline hydrochloride (DH lipomer) following intravenous route. DH lipomer was prepared by modified nano-precipitation method as reported earlier. Doxycyline loading was found to be 20 ± 2.5 %. Average particle size of DH lipomer and blank lipomer was 512 ± 8 and 520 ± 6 nm, respectively. Micronucleus (MN) assay was performed in adult healthy Swiss mice whereas chromosomal aberration (CA) test and comet assay were performed in healthy Holtzman rats following intravenous administration. Animals were divided into two sets, male and female, each set comprising of six groups (n = 5/group), viz., three test groups, blank lipomer (BL), vehicle control (VC), and positive control. Groups treated with 1.5 mg/kg BW DH lipomer did not show micronuclei formation in bone marrow cell, DNA damage, and CA, respectively, as compared with VC, suggesting no genotoxicity. On the other hand 3 and 6 mg/kg BW revealed significant (P > 0...Continue Reading

References

Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A Gabizon, D Papahadjopoulos
Aug 30, 1984·Biochemical and Biophysical Research Communications·O Ostling, K J Johanson
Sep 28, 2000·Mutation Research·G KrishnaJ Paulissen
May 10, 2001·Mutation Research·R D Snyder, J W Green
Nov 12, 2002·Environmental Health Perspectives·Shosuke KawanishiKohsuke Nishino
Jul 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marcus S CookeJoseph Lunec
Jun 5, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Eunil LeeJuneyoung Lee
Feb 16, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Christie M SayesDavid B Warheit
Dec 1, 2007·Journal of Toxicology and Environmental Health. Part a·Elena R KisinAnna A Shvedova
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stephanie E A GrattonJoseph M DeSimone
Jan 20, 2009·The Journal of Antimicrobial Chemotherapy·André C AmaralMaria Sueli S Felipe
Feb 25, 2009·ACS Nano·P V AshaRaniSuresh Valiyaveettil
May 21, 2009·Journal of Pharmaceutical Sciences·Amol B MorePadma V Devarajan
Jan 22, 2010·Journal of Pharmaceutical Sciences·Padma V DevarajanAbdul Samad
May 18, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Prajakta DandekarVandana Patravale
Sep 8, 2010·Journal of Pharmaceutical Sciences·Prajakta P DandekarVandana Patravale
Jul 26, 2011·Environmental and Molecular Mutagenesis·Shuguang WangJeffrey K Wickliffe
May 3, 2012·Journal of Nanobiotechnology·Liming XuLaisheng Lee Chou

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