PMID: 9630530Jun 19, 1998Paper

Genotoxicity of coke-oven and urban air particulate matter in in vitro acellular assays coupled with 32P-postlabeling and HPLC analysis of DNA adducts

Mutation Research
Blanka BinkováRadim J Srám

Abstract

This study is an in vitro part of the ongoing biomarker studies with population from a polluted region of Northern Bohemia and coke-oven workers from Czech and Slovak Republics. The aim of this study is to compare DNA adduct forming ability of chemical compound classes from both the urban and coke-oven extractable organic mass (EOM) of airborne particles. The crude extracts were fractionated into seven fractions by acid-base partitioning and silica gel column chromatography. In in vitro acellular assays we used calf thymus DNA (CT DNA) with oxidative (+S9) and reductive activation mediated by xanthine oxidase (+XO) under anaerobic conditions. Both the butanol and nuclease P1 versions of 32P-postlabeling for detection of bulky aromatic and/or hydrophobic adducts were used. The results showed that the spectra of major DNA adducts resulting from both the in vitro assays are within the fractions similar for both the urban and coke-oven samples. The highest DNA adduct levels with S9-activation were detected for the neutral aromatic fraction, followed by slightly polar and acidic fractions for both samples. With XO-mediated metabolism, the highest DNA adduct levels were detected for both the acidic fractions. Assuming additivity of c...Continue Reading

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Citations

Jan 9, 2008·Archives of Environmental Contamination and Toxicology·Kentaro MisakiTomonari Matsuda
Oct 13, 1999·Mutation Research·R J SrámJ Dejmek
Sep 10, 2020·International Journal of Environmental Research and Public Health·Radim J Sram
Mar 31, 2004·Molecular Aspects of Medicine·Beatriz González-Flecha

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