Genotoxicity testing of extracts from aflatoxin-contaminated peanut meal, following chemical decontamination

Food Additives and Contaminants
Laurentius A P HoogenboomJ Delort-Laval

Abstract

One of the most important concerns in the decontamination of aflatoxin-containing feed commodities is the safety of the products for food-producing animals and for human consumption of products derived from these animals. A new method, based on the use of florisil and C18 solid phase extraction columns, was developed for the preparation of extracts from decontaminated peanut meal, which allowed testing with in vitro genotoxicity assays without interference of the residual aflatoxin B1. Recovery of degradation products in the extracts was evaluated by the use of radiolabelled [14C]-aflatoxin B1 (AFB1) added to naturally-contaminated peanut meal (3.5 mg AFB1/kg). The meal was treated by a small-scale version of an industrial decontamination process based on ammoniation. Following decontamination, more than 90% of the label could not be extracted from the meal. AFB1 accounted for about 10% of the radiolabel present in the extractable fraction, indicating a total AFB1 reduction of more than 99%. Decontamination of the meal by a number of other small- and industrial-scale ammonia-based processes resulted in similar efficiencies. Application of the extraction procedure resulted in AFB1-rich and AFB1-poor fractions, the latter contain...Continue Reading

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Citations

Nov 21, 2019·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Xiaoqing GuoNan Mei
Feb 24, 2001·Food Additives and Contaminants·L A HoogenboomJ Delort-Laval

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