Genotype 1 and genotype 2 bovine noroviruses are antigenically distinct but share a cross-reactive epitope with human noroviruses

Journal of Clinical Microbiology
S L OliverP R Lambden

Abstract

The bovine enteric caliciviruses Bo/Jena/1980/DE and Bo/Newbury2/1976/UK represent two distinct genotypes within a new genogroup, genogroup III, in the genus Norovirus of the family Caliciviridae. In the present study, the antigenic relatedness of these two genotypes was determined for the first time to enable the development of tests to detect and differentiate between both genotypes. Two approaches were used. First, cross-reactivity was examined by enzyme-linked immunosorbent assay (ELISA) using recombinant virus-like particles (VLPs) and convalescent-phase sera from calves infected with either Jena (genotype 1) or Newbury2 (genotype 2). Second, cross-reactivity was examined between the two genotypes with a monoclonal antibody, CM39, derived using Jena VLPs. The two genotypes, Jena and Newbury2, were antigenically distinct with little or no cross-reactivity by ELISA to the heterologous VLPs using convalescent calf sera that had homologous immunoglobulin G titers of log10 3.1 to 3.3. CM39 reacted with both Jena and heterologous Newbury2 VLPs. The CM39 epitope was mapped to nine amino acids (31PTAGAQIAA39) in the Jena capsid protein, which was not fully conserved for Newbury2 (31PTAGAPVAA39). Molecular modeling showed that the ...Continue Reading

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Citations

Apr 26, 2013·Journal of Clinical Microbiology·Vipin Kumar MenonGagandeep Kang
Jun 29, 2007·Journal of Clinical Microbiology·S L OliverJ C Bridger
May 15, 2008·PloS One·Omar SalimPaul R Lambden
Jun 22, 2007·Proceedings of the National Academy of Sciences of the United States of America·Vernon K WardPaul R Lambden
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May 27, 2021·Transboundary and Emerging Diseases·Matías CastellsRodney Colina

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