Genotype-activity relationship for Mn-superoxide dismutase, glutathione peroxidase 1 and catalase in humans

Pharmacogenetics and Genomics
Maria BastakiNina Holland

Abstract

This study examined the association between genetic polymorphisms and enzyme activity for antioxidant enzymes that share a common detoxification pathway: manganese superoxide dismutase (MnSOD), glutathione peroxidase-1 (GPX1) and catalase. MnSOD, GPX1, and catalase activities were measured in isolated erythrocytes of 231 healthy, non-smoking student volunteers (55% women, ages 17-21, majority Asian or Caucasian). DNA from blood clots was genotyped by Taqman PCR (C47T : MnSOD and C593T : GPX1) and standard PCR (-262C>T : catalase). Associations between genotype and enzyme activity were analyzed by multiple linear regression, adjusted for baseline factors including gender and ethnicity. Minor allele frequencies ranged from 13% for catalase (T) to 18% for GPX1 (T), and 33% for MnSOD(C) with significant variation between ethnicities. Median GPX1 activity was 13.2 U/g Hb with a six-fold difference between lowest and highest levels. Catalase activity ranged eight-fold (median: 86.3 k/g Hb), while median MnSOD activity was 2.8 U/mg Hb with a 56-fold range of values. MnSOD enzyme activity was 15% higher in females than males (95%CI : -1%, 32%), and 33% higher in CT or TT individuals (C47T) versus CC individuals (95%CI : 7-59%). On aver...Continue Reading

References

Jan 1, 1984·Methods in Enzymology·L Flohé, F Otting
Jan 1, 1984·Methods in Enzymology·L Flohé, W A Günzler
Jan 1, 1984·Methods in Enzymology·H Aebi
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·J S RosenblumR A Lerner
Jul 30, 1999·Biochemical and Biophysical Research Communications·S HiroiA Kimura
Aug 7, 1999·Human Heredity·G F Van LandeghemG Beckman
Dec 9, 2000·Blood Cells, Molecules & Diseases·L ForsbergR Morgenstern
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·J R Arthur
May 24, 2001·Archives of Biochemistry and Biophysics·L ForsbergR Morgenstern
Dec 6, 2001·Journal of the National Cancer Institute·L I WangD C Christiani
Mar 13, 2002·Toxicology Letters·Yun-Chul HongDavid C Christiani
Jun 7, 2002·The Journal of Biological Chemistry·Marina MattiazziGiovanni Manfredi
Oct 23, 2002·Clinical Endocrinology·Cosimo MassafraGiuseppe Buonocore
Jun 9, 2004·Free Radical Biology & Medicine·Holly Van RemmenArlan Richardson
May 3, 2005·Pharmacogenetics and Genomics·Angela SuttonFrançoise Degoul
Aug 4, 2005·Journal of Toxicology and Environmental Health. Part a·Yu-Mei HsuehChien-Jen Chen
Aug 23, 2005·Proceedings of the American Thoracic Society·William MacNee
Oct 14, 2005·Epidemiology·Ira B TagerNino Künzli

❮ Previous
Next ❯

Citations

Jan 6, 2012·Journal of Neurology·Rebecca Deering BroseKirby D Smith
Jul 17, 2012·Biochemical Genetics·Marta Wlodarczyk, Grazyna Nowicka
Oct 12, 2012·Cancer Causes & Control : CCC·Lauren E McCulloughMarilie D Gammon
Jan 1, 2010·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Vibhuti SrivastavaDavid Goldman
Nov 20, 2010·Antioxidants & Redox Signaling·Edith LubosDiane E Handy
Mar 24, 2012·Journal of Medicinal Food·Barbara TomaselloMarcella Renis
Jun 11, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sharon A GlynnStefan Ambs
Feb 1, 2013·Journal of Nutrigenetics and Nutrigenomics·Nishi KarunasingheLynnette R Ferguson
May 7, 2010·Environmental Health : a Global Access Science Source·Ana L Miranda-VilelaMaria N Klautau-Guimarães
Dec 10, 2008·Arhiv za higijenu rada i toksikologiju·Alenka FrankoMetoda Dodic-Fikfak
Feb 25, 2014·International Journal of Molecular Sciences·Azahara I RupérezConcepción M Aguilera
Nov 28, 2013·Cancer Causes & Control : CCC·Nikhil K KhankariMarilie D Gammon
Apr 21, 2012·Pharmacogenomics·Christina JustenhovenHiltrud Brauch
May 9, 2014·The Annals of Thoracic Surgery·Daniel Seung KimGail P Jarvik
Dec 3, 2014·Neurologia i neurochirurgia polska·Karolina SpisakAgnieszka Slowik
Sep 3, 2013·Free Radical Research·S Ben-ZakenY Meckel
Nov 27, 2007·Mutation Research·G IarmarcovaiT Orsière
Jul 23, 2008·Cell Biochemistry and Function·Zorica Arsova-SarafinovskaAleksandar J Dimovski
Sep 30, 2010·American Journal of Human Biology : the Official Journal of the Human Biology Council·Ana Luisa Miranda-VilelaCesar K Grisolia
Oct 4, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Lin HanWon Sang Park
Sep 10, 2015·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A V PolonikovM A Solodilova
Dec 2, 2014·Oxidative Medicine and Cellular Longevity·Claudia BănescuMinodora Dobreanu
Dec 6, 2011·Mitochondrion·Gerassimos LascaratosAnthony H V Schapira
Mar 19, 2013·Mechanisms of Ageing and Development·Lisa S NolanSally J Dawson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.