Genotype and biotype of invasive Anopheles stephensi in Mannar Island of Sri Lanka

Parasites & Vectors
S N SurendranR Ramasamy

Abstract

Anopheles stephensi, the major vector of urban malaria in India, was recently detected for the first time in Sri Lanka in Mannar Island on the northwestern coast. Since there are different biotypes of An. stephensi with different vector capacities in India, a study was undertaken to further characterise the genotype and biotype of An. stephensi in Mannar Island. Mosquito larvae were collected in Pesalai village in Mannar and maintained in the insectary until adulthood. Adult An. stephensi were identified morphologically using published keys. Identified adult An. stephensi were molecularly characterized using two mitochondrial (cox1 and cytb) and one nuclear (ITS2) markers. Their PCR-amplified target fragments were sequenced and checked against available sequences in GenBank for phylogenetic analysis. The average spiracular and thoracic lengths and the spiracular index were determined to identify biotypes based on corresponding indices for Indian An. stephensi. All DNA sequences for the Mannar samples matched reported sequences for An. stephensi from the Middle East and India. However, a single nucleotide variation in the cox1 sequence suggested an amino acid change from valine to methionine in the cox1 protein in Sri Lankan An....Continue Reading

References

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Citations

Jan 15, 2020·Parasites & Vectors·Christian MitriMichelle M Riehle
Sep 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·M E SinkaK J Willis
Jan 27, 2021·Emerging Infectious Diseases·Fitsum G TadesseTeun Bousema

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Datasets Mentioned

BETA
MF124611
MF975722–28
DQ310148
AY702485
HQ703001
KT382827
KT921409
KF406660
KJ462218
FJ526585

Methods Mentioned

BETA
PCR

Software Mentioned

DnaSP
BLASTn
MEGA
Clustal W
BLASTP

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