Genotype-dependent serologic reactivities in patients infected with hepatitis C virus in the United States

Mayo Clinic Proceedings
N N ZeinD H Persing

Abstract

To evaluate the serologic reactivities in patients infected with different hepatitis C virus (HCV) genotypes to four HCV proteins that are components of the second-generation recombinant immunoblot assay. Serum samples from 36 patients with chronic HCV infection were obtained. RNA was extracted by using chaotropic lysis and isopropanol precipitation. Reverse-transcriptase polymerase chain reaction of the NS-5 region was performed, followed by automated single-pass dideoxy sequencing of desalted amplification products. Classification of isolated HCV subtypes was based on Simmonds' system. All samples were tested for antibodies to proteins 5-1-1, C100-3, C33c, and C22-3 with the second-generation recombinant immunoblot assay. Reactivity to protein 5-1-1 was significantly lower for patients with genotypes 2b and 3a than for those infected with HCV types 1a or 1b (P < 0.05). Antibody reactivity to the C100-3 protein was also reduced in patients infected with HCV types 2b and 3a. These data indicate that the genotype-dependent differences in serologic reactivities are substantial among patients with chronic HCV infection.

References

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Nov 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H T ChungA S Lok
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Citations

Feb 7, 2001·Clinical Microbiology Reviews·N N Zein
Oct 6, 1997·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·G LodiS R Porter
Nov 27, 2002·Transplantation·Violet A KelleyFrederick S Nolte
Oct 30, 1998·Journal of Gastroenterology and Hepatology·S KabaG C Farrell
Feb 1, 1996·Digestive Diseases and Sciences·A Czaja, H A Carpenter
Dec 17, 1997·Journal of Clinical Microbiology·J A NevilleP Simmonds
Jun 2, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Madhavi ChandraMohammed N Khaja
May 13, 1999·Mayo Clinic Proceedings·N N ZeinA J Czaja

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