Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine

Scientific Reports
Yi-Chin FanShyan-Song Chiou

Abstract

Swine are a critical amplifying host involved in human Japanese encephalitis (JE) outbreaks. Cross-genotypic immunogenicity and sterile protection are important for the current genotype III (GIII) virus-derived vaccines in swine, especially now that emerging genotype I (GI) JE virus (JEV) has replaced GIII virus as the dominant strain. Herein, we aimed to develop a system to generate GI JEV virus-like particles (VLPs) and evaluate the immunogenicity and protection of the GI vaccine candidate in mice and specific pathogen-free swine. A CHO-heparan sulfate-deficient (CHO-HS(-)) cell clone, named 51-10 clone, stably expressing GI-JEV VLP was selected and continually secreted GI VLPs without signs of cell fusion. 51-10 VLPs formed a homogeneously empty-particle morphology and exhibited similar antigenic activity as GI virus. GI VLP-immunized mice showed balanced cross-neutralizing antibody titers against GI to GIV viruses (50% focus-reduction micro-neutralization assay titers 71 to 240) as well as potent protection against GI or GIII virus infection. GI VLP-immunized swine challenged with GI or GIII viruses showed no fever, viremia, or viral RNA in tonsils, lymph nodes, and brains as compared with phosphate buffered saline-immunize...Continue Reading

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Citations

Aug 3, 2019·PLoS Neglected Tropical Diseases·Jianchao WeiZhiyong Ma
Dec 13, 2019·Science Translational Medicine·Jody Hobson-PetersRoy A Hall
Nov 7, 2019·Veterinary Research·Santosh Dhakal, Gourapura J Renukaradhya

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
transmission electron microscopy
ELISA

Software Mentioned

GraphPad Prism

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