Genotype-specific differences in structural features of hepatitis C virus (HCV) p7 membrane protein

Biochimica Et Biophysica Acta
Monoj Mon KalitaWolfgang B Fischer

Abstract

The 63 amino acid polytopic membrane protein, p7, encoded by hepatitis C virus (HCV) is involved in the modulation of electrochemical gradients across membranes within infected cells. Structural information relating to p7 from multiple genotypes has been generated in silico (e.g. genotype (GT) 1a), as well as obtained from experiments in form of monomeric and hexameric structures (GTs 1b and 5a, respectively). However, sequence diversity and structural differences mean that comparison of their channel gating behaviour has not thus far been simulated. Here, a molecular model of the monomeric GT 1a protein is optimized and assembled into a hexameric bundle for comparison with both the 5a hexamer structure and another hexameric bundle generated using the GT 1b monomer structure. All bundles tend to turn into a compact structure during molecular dynamics (MD) simulations (Gromos96 (ffG45a3)) in hydrated lipid bilayers, as well as when simulated at 'low pH', which may trigger channel opening according to some functional studies. Both GT 1a and 1b channel models are gated via movement of the parallel aligned helices, yet the scenario for the GT 5a protein is more complex, with a short N-terminal helix being involved. However, all bun...Continue Reading

References

Jan 6, 1997·Virology·F J Van kuppeveldJ R Doedens
Apr 1, 1997·FEBS Letters·A L GriceM S Sansom
Mar 1, 1985·Physical Review A: General Physics·W G Hoover
May 4, 2002·Biochimica Et Biophysica Acta·Wolfgang B Fischer, Mark S P Sansom
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Davor PavlovićNicole Zitzmann
Sep 16, 2003·FEBS Letters·Maria Eugenia Gonzalez, Luis Carrasco
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Akito SakaiJens Bukh
Jan 20, 2006·Journal of Medicinal Chemistry·George PatargiasWolfgang B Fischer
Aug 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Wei LuBing Sun
Oct 13, 2006·The Journal of Biological Chemistry·Dean ClarkeDavid Rowlands
Jan 11, 2007·The Journal of Chemical Physics·Giovanni BussiMichele Parrinello
Jun 1, 2007·Journal of Virology·Christopher T JonesCharles M Rice
Jul 31, 2007·PLoS Pathogens·Eike SteinmannThomas Pietschmann
Aug 27, 2009·Molecular Membrane Biology·George PatargiasWolfgang B Fischer
Jul 30, 2010·The Journal of Biological Chemistry·Roland MontserretFrançois Penin
Apr 27, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Toshana L FosterStephen Griffin
Jul 4, 2012·Nature Reviews. Microbiology·José Luis NievaLuis Carrasco
Sep 11, 2013·Nature Reviews. Microbiology·Brett D Lindenbach, Charles M Rice
Sep 12, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Toshana L FosterStephen Griffin
Sep 24, 2013·Biochimica Et Biophysica Acta·Bo OuYang, James J Chou
Oct 12, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Donald B SmithPeter Simmonds

❮ Previous
Next ❯

Citations

Jan 26, 2016·Biochimica Et Biophysica Acta·Wolfgang B FischerDieter Heermann
Apr 16, 2016·Biochimica Et Biophysica Acta·Monoj Mon Kalita, Wolfgang B Fischer
Jun 10, 2015·Current Opinion in Virology·Stanley J Opella
Apr 26, 2016·The Journal of Physical Chemistry. B·Siladitya Padhi, U Deva Priyakumar
Jun 4, 2016·Computational Biology and Chemistry·Niklas LaaschWolfgang B Fischer
Jun 9, 2016·Biophysical Journal·Ulrike BreitingerHans-Georg A Breitinger
Feb 24, 2016·Molecular BioSystems·Meng-Han LinWolfgang B Fischer
Dec 19, 2017·Chemical Biology & Drug Design·Sophie L DahlWolfgang B Fischer
Aug 11, 2015·Viruses·Vanesa Madan, Ralf Bartenschlager
Oct 20, 2018·Nature·Benjamin P OestringerJason R Schnell
Jun 12, 2019·Scientific Reports·Benjamin P OestringerNicole Zitzmann
Mar 13, 2021·The Journal of General Virology·Ulrike BreitingerHans-Georg Breitinger
Sep 6, 2018·Current Opinion in Virology·Jodi A Hadden, Juan R Perilla
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shukun WeiBo OuYang

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR

Software Mentioned

VMD
ClustalX
AMBER94
MOE
Origin

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.