Genotypes, nt 1858 variants, and geographic origin of hepatitis B virus--large-scale analysis using a new genotyping method

The Journal of Infectious Diseases
M LindhA Gusdal

Abstract

The nucleotide at position 1858 of hepatitis B virus has importance in chronic hepatitis B (HB) because a cytosine at nt 1858 effectively prevents virus escape through the precore TAG stop codon mutation. The relatedness between nt 1858 and genotypes was analyzed using a new genotyping method based on restriction fragment length polymorphism (RFLP) analysis of an S gene amplicon. Seventy-three gene bank sequences were analyzed by phylogenetic tree construction and RFLP prediction. A tree supporting the existence of 6 genotypes (A-F) was obtained, with C-1858 found in 9 of 9 A genotypes, 0 of 7 B, 0 of 19 C, 1 of 10 D, 0 of 2 E, and 3 of 3 F. Serum samples from 187 HB e antigen-positive chronic carriers were analyzed: Genotypes in northern Europeans were 60% A, 31% D; in southern Europeans and Middle Easterners 96% D; in Africans 53% A, 27% D, 20% E; and in East Asians 14% A, 43% B, 43% C. Cytosine at nt 1858 was found in 36 of 44 A, 0 of 32 B, 8 of 34 C, 0 of 59 D, 0 of 3 E, and 1 of 1 F genotype samples.

Citations

Nov 14, 2000·Journal of Medical Virology·A L Ballard, E H Boxall
Sep 5, 2002·Journal of Medical Virology·Sheila A TelesClara F T Yoshida
Dec 18, 2001·Journal of Medical Virology·U DumpisP Karayiannis
Jan 10, 2002·Journal of Medical Virology·J E van SteenbergenJ A R van den Hoek
Mar 27, 2002·Journal of Biomedical Science·C J LiuD S Chen
Sep 21, 2010·Medical Microbiology and Immunology·Stefan ReuterDieter Häussinger
Oct 14, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A I ShararaR Shatila
Dec 12, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S RamiaZ Mahfoud
Jun 23, 2006·Virus Genes·Krzysztof Piotr BielawskiAnna Jadwiga Podhajska
Nov 11, 2010·Hepatology International·Shu-Fen LiuWan-Long Chuang
Jun 1, 2011·Indian Journal of Virology : an Official Organ of Indian Virological Society·Ajay KumarBalesh Kumar Tiwari
Dec 6, 2003·Journal of Virological Methods·Sonia Rodríguez-NóvoaBenito J Regueiro
Mar 29, 2005·Microbes and Infection·Hooi Tin OngHeng Fong Seow
Aug 2, 2003·Gastroenterology·Chi-Jen ChuAnna S F Lok
Aug 7, 1998·Journal of Virological Methods·M LindhP Horal
Dec 22, 1999·Journal of Virological Methods·R JardiJ Guardia
Dec 29, 2000·Journal of Hepatology·J H KaoD S Chen
Dec 12, 2001·Journal of Hepatology·T SakaiY Tameda
Feb 7, 2002·Journal of Hepatology·Jia-Horng KaoDing-Shinn Chen
Aug 15, 2001·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·W J KoM Y Lai
Mar 20, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·A M BozdayiO Uzunalimoğlu
Dec 11, 2002·Journal of Gastroenterology and Hepatology·S K SarinR Chauhan
Dec 11, 2002·Journal of Gastroenterology and Hepatology·Stephen A Locarnini
Jul 9, 2002·Journal of Gastroenterology and Hepatology·Jia-Horng Kao
Jul 9, 2002·Journal of Gastroenterology and Hepatology·Fuminaka SugauchiMasashi Mizokami
Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G K LauS Lam
Oct 5, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S J Hadziyannis, D Vassilopoulos
Oct 5, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J H KaoD S Chen
Mar 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hideaki KatoMasashi Mizokami
May 1, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chi-Jen Chu, Anna S F Lok
May 6, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Perumal VivekanandanThenmozhi Subramaniam
Sep 19, 2003·AIDS Research and Human Retroviruses·Mayte Pérez-OlmedaVincent Soriano

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