Genotypic and phenotypic characterization of enterotoxigenic Escherichia coli strains isolated from Peruvian children.

Journal of Clinical Microbiology
F P RiveraC F Lanata

Abstract

Enterotoxigenic Escherichia coli (ETEC) is a major cause of childhood diarrhea. The present study sought to determine the prevalence and distribution of toxin types, colonization factors (CFs), and antimicrobial susceptibility of ETEC strains isolated from Peruvian children. We analyzed ETEC strains isolated from Peruvian children between 2 and 24 months of age in a passive surveillance study. Five E. coli colonies per patient were studied by multiplex real-time PCR to identify ETEC virulence factors. ETEC-associated toxins were confirmed using a GM1-based enzyme-linked immunosorbent assay. Confirmed strains were tested for CFs by dot blot assay using 21 monoclonal antibodies. We analyzed 1,129 samples from children with diarrhea and 744 control children and found ETEC in 5.3% and 4.3%, respectively. ETEC was more frequently isolated from children >12 months of age than from children <12 months of age (P < 0.001). Fifty-two percent of ETEC isolates from children with diarrhea and 72% of isolates from controls were heat-labile enterotoxin (LT) positive and heat-stable enterotoxin (ST) negative; 25% and 19%, respectively, were LT negative and ST positive; and 23% and 9%, respectively, were LT positive and ST positive. CFs were id...Continue Reading

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Citations

May 31, 2012·Infection and Immunity·Felipe Del CantoRoberto Vidal
Mar 22, 2014·Japanese Journal of Infectious Diseases·Shahram NazarianShakiba Darvish Alipoor
Jul 16, 2015·Transactions of the Royal Society of Tropical Medicine and Hygiene·Anicia M MedinaJoaquim Ruiz
Dec 24, 2014·Diagnostic Microbiology and Infectious Disease·Haijian PanJianghong Meng
May 4, 2012·Expert Review of Vaccines·Ann-Mari Svennerholm, Anna Lundgren
Jul 3, 2015·PloS One·Enriqueta García-GutiérrezJesús García-Martínez
Jul 1, 2020·PLoS Neglected Tropical Diseases·Ana E Farfán-GarcíaOscar G Gómez-Duarte
Jan 24, 2017·Frontiers in Cellular and Infection Microbiology·Felipe Del CantoRoberto Vidal

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