Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage

Animal Science Journal = Nihon Chikusan Gakkaihō
Masanori TohnoYimin Cai

Abstract

Twenty-three lactic acid bacteria (LAB) isolated from three cultivars (Akiaoba, Nagahahikari and Tachiwase) of Italian ryegrass (Lolium multiflorum Lam.) silage were precisely characterized by a combination of phenotypic tests, genotypic 16S ribosomal DNA sequencing and rapid PCR-based analyses, focusing on their useful phenotypes for silage preparation as inoculants. We successfully identified both at the species and subspecies levels: phenotypically novel Lactococcus lactis subsp. lactis, Lactobacillus brevis, Lactobacillus coryniformis subsp. torquens, Lactobacillus curvatus, Lactobacillus plantarum subsp. plantarum, Lactobacillus sakei subsp. carnosus, Leuconostoc mesenteroides subsp. dextranicum and Pediococcus parvulus. This is the first report to elucidate the presence of Lactobacillus coryniformis ssp. torquens and Leuconostoc mesenteroides subsp. dextranicum in Italian ryegrass silages. Physiological and biochemical tests revealed that phenotypic characteristics are different among the different strains of the same species and subspecies, and that the isolates show unique and diverse phenotypes related to fermentation factors, such as available carbohydrates, optimal growth pH and temperature. These results suggest tha...Continue Reading

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Citations

Jul 31, 2012·International Journal of Systematic and Evolutionary Microbiology·Masanori TohnoKiyoshi Tajima
Aug 8, 2013·International Journal of Systematic and Evolutionary Microbiology·Masanori TohnoKiyoshi Tajima
Mar 27, 2015·International Journal of Systematic and Evolutionary Microbiology·Masanori TohnoKiyoshi Tajima
Feb 5, 2013·International Journal of Systematic and Evolutionary Microbiology·Masanori TohnoKiyoshi Tajima
Dec 12, 2012·International Journal of Systematic and Evolutionary Microbiology·Masanori TohnoKiyoshi Tajima
May 21, 2013·International Journal of Systematic and Evolutionary Microbiology·Masanori TohnoKiyoshi Tajima
Aug 5, 2014·International Journal of Food Microbiology·Luís AbrunhosaArmando Venâncio
May 1, 2015·Asian-Australasian Journal of Animal Sciences·Dongxia LiQingsheng Jin
Jul 7, 2016·International Journal of Systematic and Evolutionary Microbiology·Masanori TohnoKiyoshi Tajima
Oct 17, 2017·International Journal of Systematic and Evolutionary Microbiology·Hisami KobayashiMasanori Tohno
Dec 13, 2018·International Journal of Systematic and Evolutionary Microbiology·Hisami KobayashiMasanori Tohno
Sep 8, 2017·International Journal of Systematic and Evolutionary Microbiology·Masanori TohnoHisami Kobayashi
Mar 14, 2017·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)Pablo Salvador Fernández Escámez
Jul 16, 2021·International Journal of Systematic and Evolutionary Microbiology·Masanori TohnoHisami Kobayashi
Aug 12, 2021·International Journal of Systematic and Evolutionary Microbiology·Hisami KobayashiMasanori Tohno
Nov 9, 2021·International Journal of Systematic and Evolutionary Microbiology·Hisami KobayashiMasanori Tohno

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