Genotypic and phenotypic diversity in tropical strains of Aspergillus spp. (section Circumdati) isolated from insects

Current Microbiology
Aurea MoraesViviane Zahner

Abstract

The morphology, multilocus enzyme electrophoresis (MLEE), and RAPD-PCR profiles of a panel of 63 strains of Aspergilus section Circumdati, all isoloated from Brazilian insects, were examined. When compared to the descriptions reported in the literature, differences were observed in terms of colony diameter for the representatives studies. Numerical taxonomy based on data generated by MLEE identified two distinct subgroups among the A. ochraceus isolates. In addition, phosphoglucose isomerase (GPI-1) was detected only in A. sclerotiorum, while phosphofructokinase (FK-1) and acid phosphatase (ACP-2) were present only in strains of A. sulphureus, suggesting that these alleles (bands) could be used for species-specific detection. Using RAPD-PCR, species-specific molecular markers were identified for both A. petrakii and A. sulphureus. These results are important from the taxonomic viewpoint and may also be used in the design of screening programs for the isoloation of new strains.

References

Jul 1, 1976·Annals of Human Genetics·Y H EdwardsH Harris
Jul 10, 1999·Clinical Microbiology Reviews·J GuarroA M Stchigel
Aug 10, 2000·Canadian Journal of Microbiology·J VargaR F Hoekstra
Jan 1, 1953·Advances in Genetics·G PONTECORVOA W J BUFTON

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