Genotypic and Phenotypic Diversity of Herpes Simplex Virus 2 within the Infected Neonatal Population

MSphere
Lisa N AkhtarMoriah L Szpara

Abstract

More than 14,000 neonates are infected with herpes simplex virus (HSV) annually. Approximately half display manifestations limited to the skin, eyes, or mouth (SEM disease). The rest develop invasive infections that spread to the central nervous system (CNS disease or encephalitis) or throughout the infected neonate (disseminated disease). Invasive HSV disease is associated with significant morbidity and mortality, but the viral and host factors that predispose neonates to these forms are unknown. To define viral diversity within the infected neonatal population, we evaluated 10 HSV-2 isolates from newborns with a range of clinical presentations. To assess viral fitness independently of host immune factors, we measured viral growth characteristics in cultured cells and found diverse in vitro phenotypes. Isolates from neonates with CNS disease were associated with larger plaque size and enhanced spread, with the isolates from cerebrospinal fluid (CSF) exhibiting the most robust growth. We sequenced complete viral genomes of all 10 neonatal viruses, providing new insights into HSV-2 genomic diversity in this clinical setting. We found extensive interhost and intrahost genomic diversity throughout the viral genome, including amino...Continue Reading

References

Aug 1, 1987·The Journal of General Virology·H SakaokaK Fujinaga
Jan 1, 1988·Microbial Pathogenesis·F Gonzalez-ScaranoN Nathanson
Jan 1, 1980·Annals of the New York Academy of Sciences·D M LonsdaleK G Warren
Nov 1, 1995·Journal of Virology·K S DingwellD C Johnson
Jan 1, 1995·Archives of Virology·U GeorgopoulouP Mavromara
Jul 1, 1996·Journal of Virology·H M FriedmanJ Lubinski
Mar 14, 1998·Journal of Virology·A DolanD J McGeoch
Aug 26, 1998·The Journal of General Virology·H YamadaY Nishiyama
Dec 10, 1999·The Journal of Experimental Medicine·J LubinskiH M Friedman
Jan 5, 2001·Molecular Biology and Evolution·K StrimmerV Moulton
Jun 9, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·S Ustaçelebi
Aug 3, 2001·Pediatrics·D W KimberlinUNKNOWN National Institute of Allergy and Infectious Diseases Collaborative Antiviral Study Group
Aug 3, 2001·Pediatrics·D W KimberlinUNKNOWN National Institute of Allergy and Infectious Diseases Collaborative Antiviral Study Group
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Jan 9, 2003·JAMA : the Journal of the American Medical Association·Zane A BrownLawrence Corey
May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Rory BowdenRyk Ward
Dec 27, 2005·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Rory BowdenPeter Donnelly
Dec 28, 2005·Journal of Virology·Mercy E OkoyePrashant Desai
Jan 18, 2006·Journal of Virology·Walt E AdamsonFrazer J Rixon
Mar 10, 2007·Virology·Maria H Lymberopoulos, Angela Pearson
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Sep 18, 2007·Science·Shen-Ying ZhangJean-Laurent Casanova
Sep 21, 2007·Journal of Virology·Peter NorbergJan-Ake Liljeqvist
Feb 17, 2009·The Journal of General Virology·Jan Åke LiljeqvistPeter Norberg
Sep 24, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Peter Norberg
Oct 3, 2009·The New England Journal of Medicine·Lawrence Corey, Anna Wald
Jan 15, 2010·The Journal of General Virology·Gabriel André Leiva-TorresAngela Pearson

❮ Previous
Next ❯

Citations

Oct 5, 2019·Cold Spring Harbor Molecular Case Studies·Mackenzie M ShipleyMoriah L Szpara
Apr 8, 2020·Current Protocols in Microbiology·Daniel P Depledge, Angus C Wilson
Dec 12, 2021·Journal of the Pediatric Infectious Diseases Society·Lisa N Akhtar, David W Kimberlin

❮ Previous
Next ❯

Datasets Mentioned

BETA
GTX100118

Methods Mentioned

BETA
PCR
Illumina sequencing

Software Mentioned

EVOS
SnpSift
MEGA
Viral Genome Assembly ( VirGA )
UPenn
HTSeq
MAFFT
ImageJ
MEGA Omega
SnpEff

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.