Genotypic and phenotypic heterogeneity in Enterococcus isolates from Batzos, a raw goat milk cheese

International Journal of Food Microbiology
L PsoniE Litopoulou-Tzanetaki

Abstract

This study investigated the genotypic and phenotypic diversity in 34 isolates of enterococci obtained during ripening of Batzos cheese from raw goat milk and characterized phenotypically as Enterococcus durans. RAPD-PCR, plasmid profiling and PFGE were used to study the genetic variability and distinguish closely related isolates. Species recognition by means of RAPD-PCR was in agreement with the phenotypic identification for 29 strains. One strain was characterized as Lactococcus lactis subsp. lactis by RAPD-PCR and four strains were grouped with the Enterococcus faecium reference strain. All strains were vancomycin sensitive, while 10 strains showed beta-haemolytic reaction on human blood and the majority of them (88.9%) showed decarboxylase activity on tyramine. All strains exhibited antagonistic activity against Bacillus cereus, Staphylococcus aureus, Escherichia coli and Listeria monocytogenes and the majority inhibited Enterococcus faecalis. Isolates displayed weak acidifying ability and low proteolytic activities when grown in milk for 24h. However, their caseinolytic activity after growth in milk for seven days was significant with preference for alphas-casein degradation.

Citations

Oct 18, 2006·Applied Microbiology and Biotechnology·María FernándezMiguel A Alvarez
Feb 12, 2010·International Journal of Microbiology·Stefano MorandiRoberta Lodi
Apr 21, 2009·International Journal of Food Microbiology·Antonio M Martín-PlateroManuel Martínez-Bueno
Mar 10, 2009·Food Microbiology·Antonio M Martín-PlateroManuel Martínez-Bueno
Jan 24, 2007·International Journal of Food Microbiology·L PsoniE Litopoulou-Tzanetaki
Feb 6, 2008·Journal of Basic Microbiology·Gustavo Pelicioli RiboldiJeverson Frazzon
Jul 29, 2015·Journal of Applied Microbiology·I JaouaniC C G Silva
Aug 6, 2015·Journal of Food Science and Technology·Leila Bousmaha-Marroki, Ahmed Marroki
May 25, 2016·Applied Microbiology and Biotechnology·Nivia CárdenasLeonides Fernández
Sep 8, 2016·The Journal of Dairy Research·Stefano MorandiMilena Brasca
May 8, 2015·Journal of Food Protection·Fang LiuWeimin Xu
Aug 31, 2014·Applied and Environmental Microbiology·Magdalena Ruiz-RodríguezManuel Martínez-Bueno
Jul 7, 2020·Microbial Pathogenesis·Likaa Hamied MahdiIbtesam Ghadban Auda
Feb 1, 2014·Microbiology Spectrum·Evanthia Litopoulou-Tzanetaki, Nikolaos Tzanetakis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacillus Cereus Infection

Bacillus Cereus is a gram-positive bacteria that is the cause of some foodborne illnesses and leads to diarrhea and vomiting. Discover the latest research on Bacillus Cereus Infection here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

International Journal of Food Microbiology
M R Foulquié MorenoL De Vuyst
International Journal of Food Microbiology
Giorgio Giraffa
International Journal of Food Microbiology
Charles M A P FranzM E Stiles
FEMS Microbiology Reviews
Giorgio Giraffa
© 2021 Meta ULC. All rights reserved