Genotypic characteristics of multidrug-resistant Pseudomonas aeruginosa from hospital wastewater treatment plant in Rio de Janeiro, Brazil

Journal of Applied Microbiology
C C MirandaMaysa M Clementino

Abstract

To investigate Pseudomonas aeruginosa isolates from a hospital wastewater treatment plant (HWTP), focusing on enzyme-based mechanisms of β-lactams resistance and the genetic relatedness among isolates. Forty-one Ps. aeruginosa strains recovered from a HWTP were identified by amplification of 16S rRNA gene. β-lactamase production was screened by disc diffusion, CHROMagar extended-spectrum β-lactamase (ESBL) and β-lactamase strips. β-lactamase and ESBL producing isolates were investigated by PCR for the presence of ESBL, metallo-β-lactamase and Klebsiella pneumoniae carbapenemase encoding genes. Thirty-four isolates (83%) were resistant to at least one antibiotic belonging to three or more classes. Out of these 34 isolates, 28 (82%) were classified as multidrug-resistant (MDR) and 6 (18%) extensively drug-resistant (XDR). Genetic relatedness by Enterobacterial Repetitive Intergenic Consensus sequence-PCR and Multilocus sequence typing analysis showed 20 distinct profiles and 15 sequencing types respectively. Clonal Complex 244 (CC244) shows the pathogenic potential of this clone carrying MDR and XDR strains from clinical, environmental and hospital waste sources. Our results suggest that treatment facilities for hospital wastewat...Continue Reading

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Citations

Apr 27, 2018·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Luiza Gerçossimo OliveiraMagna Cristina Paiva
Aug 19, 2015·Journal of Applied Microbiology·D O SantoroM M Clementino
Apr 26, 2021·Journal of Global Antimicrobial Resistance·Zineb CherakJean-Marc Rolain

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