Genotypic Diversity among Campylobacter jejuni Isolates in a Commercial Broiler Flock.

Applied and Environmental Microbiology
L M ThomasP R Widders

Abstract

Analysis of nucleic acid polymorphism in the flagellin genes of Campylobacter jejuni was used to investigate genetic diversity among Campylobacter spp. in a commercial broiler flock. Three hundred single colonies of C. jejuni were isolated from fecal samples collected weekly for 3 weeks immediately before slaughter. Both the flaA and flaB genes were amplified by PCR, and the PCR product was digested with the restriction enzyme AluI. The fragments generated were then analyzed by agarose gel electrophoresis. Among the 300 recovered isolates, five different restriction fragment length polymorphism profiles were observed. Three of these profiles were dominant during the course of the study, and the other two profiles were detected at low frequency. Analysis of genetic variation in C. jejuni over the course of an experimental infection lasting 7 weeks indicated that there was no obvious drift in the flagellin gene type. These findings demonstrate that a range of bacterial genotypes can constitute the bacterial population within a commercial poultry flock, with the most likely sources of these types being multiple environmental exposure and/or genetic drift within the population. This degree of diversity must be considered in epidemi...Continue Reading

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Citations

May 3, 2007·Journal of the Royal Society, Interface·Andrew J K ConlanJulia R Gog
Mar 21, 2007·Antonie van Leeuwenhoek·Günter KleinViktoria Atanassova
Aug 7, 2003·Applied and Environmental Microbiology·D G Newell, C Fearnley
Aug 19, 2007·Folia Microbiologica·K Baldy-Chudzik, M Stosik
Jun 4, 2005·Applied and Environmental Microbiology·Omar A OyarzabalRobert S Miller
Nov 21, 2007·PLoS Pathogens·Beate SkånsengKnut Rudi
Apr 16, 2003·Journal of Applied Microbiology·I CoolsJ Debevere

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