Genotypic heterogeneity and correlation to intergenic haplotype within high HbF beta-thalassemia intermedia

European Journal of Haematology
Adamantia PapachatzopoulouAglaia Athanassiadou

Abstract

A molecular study was carried out of beta-thalassemia intermedia patients, compound heterozygotes for mutations usually found in beta-thalassemia major, with high levels of HbF in the absence of hereditary persistence of fetal hemoglobin (HPFH) syndrome. Our objective was to locate cis-DNA structures, DNA haplotypes, motifs, or polymorphisms that may correlate with the presence of high HbF. Allele-specific oligonucleotide (ASO) hybridization was used for the detection of mutations and restriction fragment length polymorphism (RFLP) analysis and automated sequencing for motifs, haplotypes, and polymorphisms. Southern blot was used for investigating alpha-thalassemia and/or alpha- or gamma-globin genes triplications. RNA extracted from burst forming unit-erythroid (BFU-e) colonies of peripheral blood mononuclear cell cultures was used in reverse transcriptase-polymerase chain reaction (RT-PCR) to investigate intergenic transcription. We established that (i) the combination: T haplotype of the Agamma-delta-globin intergenic region, the motif (TA)9N10(TA)10 in the HS2 site of locus control region (LCR), and TAG pre-Ggamma haplotype is sufficient but not necessary for high HbF, (ii) the genetic determinant(s) for high HbF involves a...Continue Reading

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Citations

Dec 21, 2013·Blood Cells, Molecules & Diseases·Anita NadkarniK Ghosh
Sep 18, 2008·Hematology·Inusha Panigrahi, Sarita Agarwal
Feb 25, 2015·Hemoglobin·Jabbar KhanNaseruddin Hoti
Jul 27, 2007·American Journal of Hematology·Adamantia PapachatzopoulouGeorge P Patrinos
Apr 2, 2008·IUBMB Life·Laura Manca, Bruno Masala
Feb 16, 2008·Hemoglobin·George P Patrinos, Frank G Grosveld
Jul 21, 2010·Hemoglobin·Adamantia PapachatzopoulouAglaia Athanassiadou

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