Genotyping and characterization of CTX-M-15 -producing Klebsiella pneumoniae isolated from an Iranian hospital

Journal of Chemotherapy
Safoura DerakhshanBita Bakhshi

Abstract

The aims were to describe the genetic characterization of blaCTX-M-1 group gene in Klebsiella pneumoniae and to investigate the relationship between isolates by MLVA and PFGE. We analyzed 36 CTX-M group 1-ESBL producing K. pneumoniae. rmpA and wcaG virulence genes were identified by PCR. The genetic environment of blaCTX-M-1 was analyzed by PCR and sequencing. Plasmid replicons were determined using PCR-based replicon typing. The isolates were typed by MLVA and PFGE. All blaCTX-M-1 were blaCTX-M-15. The wcaG and rmpA were detected in 1 and 2 isolates, respectively. IncF were the most frequently detected replicons (63.88%). In all isolates, ISEcp1 was found upstream and orf477 downstream of blaCTX-M-15, IS26 was found in two isolates. MLVA identified 20 MLVA types, whereas PFGE identified 25 different profiles. The dissemination of CTX-M-15 in our isolates was due to the clonal spread of isolates and to the genetic transfer of mobile elements among unrelated strains.

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Citations

Dec 14, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Safoura DerakhshanBita Bakhshi
Oct 24, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Zeinab JafariMohammad Mehdi Feizabadi
Apr 27, 2021·Foodborne Pathogens and Disease·Janeth Pérez-GarzaNorma Heredia

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