Genotyping and phylogenic study of Acanthamoeba isolates from human keratitis and swimming pool water samples in Iran

Parasite Epidemiology and Control
Bahman Rahimi EsboeiNahid Jalallou

Abstract

Acanthamoeba keratitis cause severe corneal infection and lead to poor vision and blindness. This disease is caused by a unicellular amphizoic protozoon called Acanthamoeba spp. that present in different environments. This study aimed to represent the existence and genotyping of Acanthamoeba spp. in patients with keratitis and swimming pool water (SPW) in Tehran Province, Central Iran. In this descriptive study, 56 clinical samples were collected from patients with keratitis and 30 water samples were collected from different swimming pools in Tehran Province. All samples were examined based on the morphological and molecular techniques. The genotypes were determined by sequencing the partial of 18S rRNA gene. Of 56 clinical (corneal) and 30 environmental (SPW) samples, 30.3% and 40.0% were positive for Acanthamoeba spp., respectively. According to sequencing analysis, 94.1% of amoebic keratitis isolates were belonged to T4 genotype and only one (5.8%) isolate was belonged to T11 genotype. All genotypes were detected from SPW samples were identified as T4 genotype. According to our results, use of contact lens and swimming in pool poses the major risk factor for amoebic keratitis in the studied area (Tehran). Moreover, T4 genoty...Continue Reading

Citations

Aug 6, 2021·Parasitology Research·Maria Luisa Nunes DiehlMarilise Brittes Rott

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Methods Mentioned

BETA
scrapings
PCR
genotyping

Software Mentioned

molecular evolutionary genetic analysis ( MEGA )
MEGA
BLAST

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