Genotyping by Amplified Fragment Length Polymorphism and malate metabolism performances of indigenous Oenococcus oeni strains isolated from Primitivo wine

International Journal of Food Microbiology
Maria Stella CappelloG Zapparoli

Abstract

The Amplified Fragment Length Polymorphism (AFLP) technique was applied for the first time to investigate the genotyping of Oenococcus oeni, the most important species involved in malolactic fermentation (MLF) in wine. A total of 87 out of 220 lactic acid bacteria, isolates from "Primitivo" wine (Apulia, Italy) undergoing MLF, identified as O. oeni by species-specific PCR and 16S rRNA sequence analysis, were studied by AFLP analysis. Four main clusters were distinguished and three of them showed intraspecific homology higher than 60%. A total of 28 strains, representative of AFLP clusters, were tested for malate metabolism in order to gain information on their malolactic performances. Significant differences were observed among strains for malic acid consumed, biomass produced and specific malic acid consumption rate. These findings indicated that AFLP technique is reliable for typing O. oeni strains and that, together with metabolism studies it may be used to individuate possible candidates as industrial malolactic starters.

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Citations

Dec 3, 2013·Food Microbiology·Olivier Claisse, Aline Lonvaud-Funel
Dec 31, 2008·Applied and Environmental Microbiology·Eric BilhèreAline Lonvaud-Funel
Jun 16, 2014·Food Microbiology·Maria Stella CappelloGiacomo Zapparoli
Aug 22, 2014·Applied Microbiology and Biotechnology·Krista M SumbyVladimir Jiranek
Mar 2, 2016·World Journal of Microbiology & Biotechnology·Carmela GarofaloVittorio Capozzi
Aug 4, 2009·Journal of Microbiological Methods·Camilla LazziErasmo Neviani
Nov 2, 2015·Microbiological Research·Vittorio CapozziGiuseppe Spano
Mar 1, 2012·Food Microbiology·Olivier Claisse, Aline Lonvaud-Funel
Jan 26, 2017·Frontiers in Microbiology·Vania C LiuzziGiuseppina Mulè
Jan 30, 2018·Journal of Applied Microbiology·M G BonomoG Salzano
Feb 23, 2019·Applied Microbiology and Biotechnology·Marc P G Lorentzen, Patrick M Lucas
Aug 9, 2011·Journal of Industrial Microbiology & Biotechnology·Melissa L Ivey, Trevor G Phister
Apr 17, 2019·Journal of Applied Genetics·Gaia BertaniCamilla Lazzi
Aug 24, 2017·FEMS Microbiology Reviews·Eveline J Bartowsky
Apr 29, 2014·Journal of Proteome Research·Anna NapoliGiovanni Sindona

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