Genotyping by Sequencing of 393 Sorghum bicolor BTx623 × IS3620C Recombinant Inbred Lines Improves Sensitivity and Resolution of QTL Detection

G3 : Genes - Genomes - Genetics
Wenqian KongAndrew H Paterson

Abstract

We describe a genetic map with a total of 381 bins of 616 genotyping by sequencing (GBS)-based SNP markers in a F6-F8 recombinant inbred line (RIL) population of 393 individuals derived from crossing S. bicolor BTx623 to S. bicolor IS3620C, a guinea line substantially diverged from BTx623. Five segregation distorted regions were found with four showing enrichment for S. bicolor alleles, suggesting possible selection during formation of this RIL population. A quantitative trait locus (QTL) study with this number of individuals, tripled relative to prior studies of this cross, provided resources, validated previous findings, and demonstrated improved power to detect plant height and flowering time related QTL relative to other published studies. An unexpected low correlation between flowering time and plant height permitted us to separate QTL for each trait and provide evidence against pleiotropy. Ten non- random syntenic regions conferring QTL for the same trait suggest that those QTL may represent alleles at genes functioning in the same manner since the 96 million year ago genome duplication that created these syntenic relationships, while syntenic regions conferring QTL for different trait may suggest sub-functionalization af...Continue Reading

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Citations

Aug 23, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Moctar KanteWillmar L Leiser
Sep 11, 2020·G3 : Genes - Genomes - Genetics·Wenqian KongAndrew H Paterson
Jan 11, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·WenQian KongAndrew H Paterson
Aug 14, 2021·PloS One·WenQian KongAndrew H Paterson
Nov 6, 2021·Planta·Zhanguo XinGeoffrey Morris

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Methods Mentioned

BETA
genotyping

Software Mentioned

Tassel GBS
qtl
TASSEL
R
QTL package
GBS

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