Genotyping of a homogeneous group of Yersinia pestis strains isolated in the United States.

Journal of Clinical Microbiology
Xiao-Zhe HuangLuther E Lindler

Abstract

Yersinia pestis, the causative agent of deadly plague, is considered a reemerging infectious disease and a significant biological terrorism threat. The present project focused on epidemiological investigation of the genetic variability of well-documented strains of Y. pestis from the United States by pulsed-field gel electrophoresis (PFGE) and restriction fragment length polymorphism (RFLP) analysis with insertion sequences IS100 and IS285 as probes. We examined 37 U.S. Y. pestis strains and isolates of a single ribotype, ribotype B, recovered between 1939 and 1998 from patients, animals, and fleas. Our results showed that all isolates had similar PFGE patterns, but minor differences such as missing, additional, and shifted bands were found among almost all strains if they came from different parent strains. The 37 strains and isolates were divided into 26 PFGE types. RFLP analysis with IS100 as a probe divided these strains and isolates into 16 types, with 43% belonging to IS100 type 1. Typing with IS285 as a probe was less specific and led to only four RFLP types, with 81% belonging to type 1. Similarity analysis with BioNumerics software showed that all strains shared >or=80, 86, and 91% similarities on dendrograms prepared ...Continue Reading

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Citations

Apr 16, 2004·Clinical Microbiology Reviews·Andrey P AnisimovGerald B Pier
Nov 10, 2006·Journal of Clinical Microbiology·Johannes Elias, Ulrich Vogel
May 8, 2004·Journal of Clinical Microbiology·Matthew M FerrisBabetta L Marrone
Feb 8, 2005·Journal of Clinical Microbiology·Jennifer L LowellKenneth L Gage
Oct 9, 2004·Annual Review of Entomology·Kenneth L Gage, Michael Y Kosoy
Sep 21, 2011·PLoS Neglected Tropical Diseases·Amy J VoglerPaul Keim
Jan 15, 2013·Genetics and Molecular Research : GMR·M P S BarrosT C Leal-Balbino
Sep 6, 2013·Molekuliarnaia genetika, mikrobiologiia i virusologiia·M E PlatonovA P Anisimov
Jan 19, 2016·Diagnostic Microbiology and Infectious Disease·Luke C KingryJeannine M Petersen
Nov 1, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Amy J VoglerDavid M Wagner
Jun 10, 2006·Infectious Disease Clinics of North America·Stephen A Morse, Bruce Budowle
Feb 13, 2016·PLoS Neglected Tropical Diseases·Laurel B Respicio-KingryJeannine M Petersen
Feb 17, 2005·Journal of Medical Microbiology·Zongzhong TongRuifu Yang

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