Genotyping of Cytomegalovirus from Symptomatic Infected Neonates in Iraq

The American Journal of Tropical Medicine and Hygiene
Sevan N AlwanBrian L Wickes

Abstract

Among all other viruses, human cytomegalovirus (HCMV) is the most frequent cause of congenital infection worldwide. Strain variation in HCMV may predict severity or outcome of congenital HCMV disease. Previous studies have associated a particular genotype with specific sequelae or more severe illness, but the results were contradictory. There are no previous studies addressing the genotype of HCMV in Iraq. Therefore, the present study is aimed at molecular detection and genotyping of HCMV isolated from symptomatic congenitally/perinatally infected neonates. This prospective study comprised 24 serum samples from symptomatic neonates with congenital/perinatal infection. Viral DNA was extracted from these serum samples; nested polymerase chain reaction was used to amplify the HCMV gB (UL55) gene. Polymerase chain reaction products of the second round of amplification were subjected to direct Sanger sequencing. Bioedit and MEGA5 software (EMBL-EBI, Hinxton, Cambridgeshire, UK) were used for alignment and construction of a phylogenetic tree. Human cytomegalovirus DNA was detected in 23 of 24 samples (95.8%). According to the phylogenetic analysis, three genotypes of the virus were identified; gB1, gB2, and gB3 genotypes. However, th...Continue Reading

References

May 29, 1998·Research in Virology·D H SheppR G Pergolizzi
Jul 18, 2000·Journal of Medical Virology·D E TrincadoW D Rawlinson
Mar 20, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·M BarbiD Melotti
Oct 12, 2001·Journal of Medical Virology·A LukácsiR Pusztai
Jun 21, 2007·Reviews in Medical Virology·Aileen Kenneson, Michael J Cannon
Jan 25, 2008·Journal of Clinical Microbiology·Zdenek NovakSuresh B Boppana
Apr 25, 2008·Current Protocols in Immunology·Eva Maria BorstMartin Messerle
Aug 14, 2009·Journal of Perinatal Medicine·Oriol CollUNKNOWN WAPM Perinatal Infections Working Group
Nov 3, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Gail J Demmler-Harrison
May 15, 2010·Current Immunology Reviews·William J MullerDavid M Koelle
Sep 3, 2010·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Giuseppina LombardiMauro Stronati
Sep 2, 2011·The Journal of Infectious Diseases·Shannon A RossSuresh B Boppana
Jan 19, 2012·Virology Journal·John Paul Tomtishen
Jun 22, 2012·Mediators of Inflammation·Lynne SykesPhillip R Bennett
Nov 13, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jutte J C de VriesAnn C T M Vossen
Nov 16, 2012·Human Vaccines & Immunotherapeutics·Devon J ShedlockDavid B Weiner
Jan 9, 2013·Clinical Microbiology Reviews·Sheetal ManicklalRavindra K Gupta
Apr 26, 2013·Vaccine·Paul GriffithsStephanie Bialek
May 23, 2013·The Pediatric Infectious Disease Journal·Sunil K PatiUNKNOWN NIDCD CHIMES Study Investigators
Jun 12, 2013·Expert Review of Vaccines·Vijayendra DasariRajiv Khanna
Jan 24, 2014·Journal of Child Neurology·Goran KrakarVlatka Mejaški Bošnjak
May 9, 2014·Viruses·Sonia M Restrepo-GualterosGustavo Nino
Jun 12, 2014·Journal of Infection in Developing Countries·José Arellano-GalindoJuan Manuel Mejía-Arangure
May 1, 2015·Pharmacology·Julia JückstockFriederike Traunmüller

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Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

MView
Bioedit
MEGA5

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