PMID: 8948130Oct 25, 1996Paper

Genotyping of five short tandem repeat loci via triplex and duplex PCR

Forensic Science International : Synergy
I RostedtA Sajantila

Abstract

We have developed a triplex PCR method for D3S1359, HumTH01 and HumTPO tetranucleotide loci and a duplex PCR method for HumFES/FPS and HumvWA31A tetranucleotide loci using high resolution polyacrylamide gel electrophoresis and silver staining. The methods were evaluated for paternity testing and individual identification and allele frequencies at these loci are reported for 189-3387 unrelated individuals in the Finnish population. The D3S1359 locus, especially, was found to be a highly informative locus. Seventeen alleles were found in the D3S1359 locus with a highest observed allele frequency of 0.199, a high exclusion power (PE) in paternity testing (0.78) and a high observed heterozygosity (0.89). The combined PE for these five loci was 0.99.

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Citations

Mar 26, 1999·American Journal of Human Genetics·T RantamäkiL Peltonen
Dec 25, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Qiongying DengXiaoxun Xie
Jun 15, 1997·Analytical Chemistry·T A Brettell, R Saferstein
Jun 29, 1999·Analytical Chemistry·T A BrettellR Saferstein

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