Genotyping of Giardia lamblia and Entamoeba spp from river waters in Iran

Parasitology Research
Mohammad Reza MahmoudiPanagiotis Karanis

Abstract

In this study, DNA from 55 surface and river water samples, which were collected from some water sources of Tehran and the Guilan Province, Iran, were extracted and examined for Entamoeba spp. and Giardia lamblia by PCR and genotyping. Twenty-seven samples, which were concentrated using the immunomagnetic separation technology (IMS) method, were examined for Giardia alone. Twenty-eight samples, which were concentrated using the sucrose flotation (SF) method, were examined for both Giardia and Entamoeba species. The results showed that 27/55 (17/27 and 10/28) (49 %), 4 /28 (14.28 %) and 3/28 (10.7 %) of the samples were positive for Giardia lamblia, Entamoeba spp and mixed infections (Entamoeba spp. and Giardia spp.), respectively. Sixteen out of 55 samples were negative. Entamoeba genus-specific PCR primers in single-round PCR were used to differentiate between the Entamoeba spp. (E. histolytica, E. dispar and E. moshkovskii). With respect to the 7 samples that were positive for Entamoeba, (14.28 %) 4 out of 28 were positive for E. moshkovskii, (7.14 %), 2 out of 28 were positive for E. histolytica and (3.57 %) 1 out of 28 was positive for E. dispar. Genus-specific PCR primers in a semi-nested PCR assay was performed to genotyp...Continue Reading

References

Aug 1, 1993·International Journal for Parasitology·P L EyG Mayrhofer
Jun 1, 1996·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·P KaranisH M Seitz
Jan 1, 1997·Clinical Microbiology Reviews·M M MarshallC R Sterling
May 10, 2003·Emerging Infectious Diseases·Ibne Karim M AliC Graham Clark
May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Carolyn M ReadR C Andrew Thompson
Jun 23, 2004·Laboratory Animals·M DorschH J Hedrich
Mar 21, 2006·Trends in Parasitology·L SavioliA Thompson
Jun 20, 2006·Environmental Research·P KaranisK Stojanova
Sep 7, 2006·Journal of Clinical Microbiology·Zulhainan HamzahPorntip Chavalitshewinkoon-Petmitr
Apr 4, 2007·Journal of Water and Health·Panagiotis KaranisHuw Smith
Oct 25, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R FotedarJ Harkness
Jun 6, 2008·Current Opinion in Biotechnology·Maha BouzidKevin M Tyler
Jul 14, 2010·International Journal of Hygiene and Environmental Health·Judit PlutzerPanagiotis Karanis
Jan 15, 2011·Clinical Microbiology Reviews·Yaoyu Feng, Lihua Xiao
Sep 4, 2012·Parasitology·Tengku Shahrul AnuarNorhayati Moktar
Jun 6, 2013·Transactions of the Royal Society of Tropical Medicine and Hygiene·Mohammad-Reza MahmoudiPanagiotis Karanis

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Citations

Jan 19, 2016·Parasitology Research·Jessica Triviño-ValenciaJorge E Gomez-Marin
Jun 10, 2016·Water Research·Judit Plutzer, Panagiotis Karanis
Jul 3, 2016·Environmental Science and Pollution Research International·Mahdi HadiRoya Zarinnejad
Oct 30, 2016·Acta Tropica·Zeynep KolorenPanagiotis Karanis
Aug 31, 2016·Environmental Technology·Priscila Ribeiro Dos Santos, Luiz Antonio Daniel
Oct 3, 2018·Journal of Environmental Quality·Kerry A HamiltonWarish Ahmed
Oct 31, 2017·Frontiers in Microbiology·Junqiang LiLongxian Zhang
Jan 23, 2020·Parasitology Research·M R MahmoudiP Karanis
Jul 9, 2020·Parasitology Research·Mohammad Reza MahmoudiPanagiotis Karanis
Aug 28, 2021·Acta Tropica·Zahra BahramdoostAli Haghighi

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