Genotyping of heterotrophic bacteria from the central baltic sea by use of low-molecular-weight RNA profiles.

Applied and Environmental Microbiology
Manfred G Höfle, Ingrid Brettar

Abstract

The Gotland Deep, an anoxic basin, was investigated for its heterotrophic microflora as a station representative of the central Baltic Sea and as an example of a brackish water environment. One hundred twenty-three bacterial strains were isolated along the water column by use of four different cultivation procedures. High-resolution electrophoresis of the low-molecular-weight (LMW) RNA (5S rRNA and tRNA) was used for analysis of the taxonomic position of the strains. The banding pattern of the LMW RNA generated by the electrophoresis allowed a taxonomic grouping at the species level of the 123 strains into 24 different genotypes. This grouping was confirmed by use of long-range gels with a substantially better resolution than that of standard gels; i.e., about 60% more tRNA bands were obtained on the long-range gels, and the distance between the bands was increased by about two-thirds. The majority of the strains (76%) could be identified to the species level by comparison with LMW RNA profiles from reference strains stored in an electronic database. Eighty-seven percent of the strains could be assigned to the families Vibrionaceae, Enterobacteriaceae, and Pseudomonadaceae (rRNA group I). The most abundant species among the iso...Continue Reading

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Citations

Jun 29, 2002·Applied and Environmental Microbiology·Katja Dominik, Manfred G Höfle
Apr 25, 2003·International Journal of Systematic and Evolutionary Microbiology·Ingrid BrettarManfred G Höfle
Jul 8, 2008·Nature Reviews. Microbiology·James K FredricksonJames M Tiedje
Nov 28, 2007·Applied and Environmental Microbiology·L RiemannA Hagström
Apr 12, 2012·Applied and Environmental Microbiology·Magnus HeunKai M Thormann
Jan 7, 1998·Electrophoresis·J M Cruz-SánchezE Martínez-Molina
Sep 28, 2012·Environmental Microbiology·Christian G BrucknerKlaus Jürgens
Sep 18, 1997·Applied and Environmental Microbiology·J PinhassiA Hagström
Jul 1, 1997·Applied and Environmental Microbiology·B Krause, K H Nealson

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