PMID: 8586059Oct 1, 1995Paper

Genotyping of human deoxyribonuclease I polymorphism by the polymerase chain reaction

Electrophoresis
T YasudaK Kishi

Abstract

We recently completely elucidated the molecular basis of genetic polymorphism in human deoxyribonuclease I and found it to be controlled by four codominant alleles, DNASE1*1, *2, *3 and *4. In this paper we describe a novel DNase I-genotyping system that could be used directly on DNA samples using the polymerase chain reaction (PCR) based on the three nucleotide substitutions underlying the protein polymorphism. The system consists of three independent reactions. Since the substitutions neither suppress nor create any known enzyme recognition site in the DNase I gene, two separate mismatched PCR followed by XhoI digestion methods were introduced to discriminate between the DNASE1*1 (or *3) and the DNASE1*2 (or *4) alleles, and to detect the DNASE1*4 allele. An amplification refractory mutation system was employed to detect DNASE1*3. A 100% correlation was found between the results of this genotyping method and those obtained by phenotyping using conventional isoelectric focusing. The high sensitivity and specificity of this genotyping method allows us to survey DNase I-polymorphism in small DNA samples.

References

Nov 1, 1992·Forensic Science International : Synergy·K SawazakiK Kishi
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D Y WuR B Wallace
May 11, 1989·Nucleic Acids Research·A HaliassosA Kitzis
Feb 1, 1989·Human Genetics·K KishiK Mizuta
Jul 1, 1971·Journal of Biomechanics·Y C Fung
Apr 1, 1995·Annals of Human Genetics·T YasudaK Kishi
Sep 17, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·T YasudaK Kishi

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Citations

Jun 28, 1996·Forensic Science International : Synergy·K Kishi, T Yasuda
Aug 26, 2003·Legal Medicine·K KishiH Takeshita
Dec 6, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Haruo TakeshitaJunko Fujihara
Apr 4, 2007·Cell Biochemistry and Function·Haruo TakeshitaTamiko Nakajima

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