Abstract
Serogroup C of Clostridium difficile is the serogroup most frequently related to outbreaks. Fifty-six toxigenic serogroup C isolates of C. difficile were genotyped by ribotyping PCR (ribo-PCR), random amplified polymorphic DNA (RAPD) assay, and pulsed-field gel electrophoresis (PFGE). Thirty-five of the 56 isolates were recovered from four unrelated outbreaks (Belgium, 1987, 1992, and 1995; France, 1992 to 1993) 7 derived from a spatiotemporal cluster in Cotonou, Benin (1992), and 14 were sporadic isolates. The serogroup C reference strain, also isolated during an outbreak (Belgium, 1983), was genotyped too. Ribo-PCR, the RAPD assay, and PFGE generated 2, 5, and 11 major genotypes, respectively. Combination of the three methods finally yielded 13 general types, although ribo-PCR did not play any role in enhancing resolution. Three general types were recovered from all the isolates from the five outbreaks and the cluster, with two types being predominant. The 14 sporadic serogroup C isolates were divided into 11 overall genotypes. These results indicate that genotyping methods, and more particularly the combination of the RAPD assay and PFGE, can resolve genetic diversity within toxigenic, serogroup C C. difficile strains. Also,...Continue Reading
References
Sep 1, 1992·The Journal of Infectious Diseases·C R ClabotsD N Gerding
Aug 1, 1992·Journal of Clinical Microbiology·J R KostmanT L Stull
Jan 1, 1990·Reviews of Infectious Diseases·S Tabaqchali
Jul 14, 1990·Lancet·S JohnsonD N Gerding
Mar 1, 1988·Journal of Clinical Microbiology·S TomaM Delmée
Jan 26, 1989·The New England Journal of Medicine·L V McFarlandW E Stamm
Feb 1, 1989·The Journal of Infectious Diseases·S JohnsonD N Gerding
Sep 1, 1988·The Journal of Antimicrobial Chemotherapy·M Delmée, V Avesani
Dec 1, 1987·European Journal of Clinical Microbiology·M DelméeJ L Michaux
Dec 1, 1986·Journal of Clinical Microbiology·M DelméeG Cornelis
Jan 1, 1986·The Journal of Infectious Diseases·S R HeardS Tabaqchali
Mar 1, 1985·Journal of Clinical Microbiology·M DelmeeG Wauters
Sep 1, 1995·Journal of Clinical Microbiology·F C TenoverB Swaminathan
Dec 1, 1993·Journal of General Microbiology·V Gürtler
Jan 1, 1995·The Journal of Infectious Diseases·J R KostmanT L Stull
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·K E BowenJ Svenson
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T V RileyC L Golledge
Aug 1, 1995·Journal of Clinical Microbiology·G MartirosianF Meisel-Mikolajczyk
Jun 1, 1995·Journal of Clinical Microbiology·A van BelkumH Koeleman
Jan 1, 1995·Journal of Clinical Microbiology·C P CartwrightV J Gill
Jun 1, 1994·Journal of Clinical Microbiology·G E Killgore, H Kato
Sep 1, 1994·Journal of Clinical Microbiology·H KatoT Abe
Oct 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A E SimorK Tsimidis
Sep 15, 1994·FEMS Microbiology Letters·E ChachatyA Andremont
Aug 1, 1994·Journal of Clinical Microbiology·M KristjánssonR D Arbeit
Jan 27, 1994·The New England Journal of Medicine·C P KellyJ T LaMont
Sep 1, 1994·The Journal of Infectious Diseases·M H SamoreA W Karchmer
May 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J G Bartlett
Aug 1, 1993·Journal of Clinical Microbiology·M J WolfhagenJ Verhoef
Feb 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M H SamoreA W Karchmer
Sep 25, 1993·Nucleic Acids Research·B Schierwater, A Ender
Dec 1, 1993·FEMS Microbiology Letters·F BarbutJ C Petit
Jun 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·K H Wilson
Mar 1, 1993·Journal of Clinical Microbiology·F BarbutJ C Petit
Jan 1, 1993·Methods in Enzymology·J G WilliamsS V Tingey
Nov 1, 1995·Journal of Clinical Microbiology·J StanleyR George
Nov 1, 1995·Journal of Clinical Microbiology·J M PrucklerB S Fields
Dec 1, 1995·Journal of Clinical Microbiology·Y J TangJ Silva
Nov 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D TalonY Michel-Briand
Citations
Jul 1, 1999·FEMS Microbiology Letters·P BidetJ C Petit
Jul 18, 2003·Journal of Bacteriology·Marie-Pierre Savariau-LacommeClaire Janoir
Apr 23, 2011·Journal of Medical Microbiology·Lauren M GreenPeter A Lambert
Nov 21, 2014·Journal of Clinical Microbiology·Alexander SchneebergChristian Seyboldt
Feb 24, 2001·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·L R ModyL L Dever
Feb 7, 2001·Microbiology·M RupnikS L Stubbs
Jul 11, 2007·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Warren N FawleyMark H Wilcox
Nov 1, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Marlene WulltMats Walder
Jul 9, 1999·FEMS Immunology and Medical Microbiology·B SpernerA Stolle
Jun 2, 2001·Electrophoresis·A StolleH Eisgruber
Jun 16, 2005·Journal of Clinical Microbiology·Warren N FawleyMark H Wilcox
Jul 6, 2000·Journal of Clinical Microbiology·P BidetJ C Petit
Sep 6, 2003·Journal of Clinical Microbiology·Bo SvenungssonThomas Akerlund
Feb 22, 2008·Journal of Clinical Microbiology·Thomas AkerlundLars G Burman
Mar 9, 2004·Journal of Clinical Microbiology·Renate J van den BergEd J Kuijper
Sep 17, 1998·Journal of Clinical Microbiology·M E RaffertyM Shayegani
Jul 17, 1998·Journal of Clinical Microbiology·M RupnikM Delmée
Apr 3, 2001·Journal of Clinical Microbiology·H KatoS Nakamura
Oct 10, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J S Brazier