DOI: 10.1101/473868Nov 19, 2018Paper

Genotyping of Salmonella spp. on the basis of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats).

BioRxiv : the Preprint Server for Biology
CALVIN JIKSINGKENNETH FRANCIS RODRIGUES

Abstract

Aims: Bacterial genotyping on the basis of the CRISPR array has been established in Mycobacterium tuberculosis with a method called spacer oligonucleotide typing (spoligotyping). The spoligotyping method had been widely used for both detection and typing of M. tuberculosis complex bacteria. This present study aimed at determining if the CRISPR array in Salmonella spp. could be applied to establish a correlationship between serogroup and the fingerprint generated by CRISPR typing. Methodology and results: A total of 30 samples were obtained from Diagnostic Veterinary Laboratory, Kota Kinabalu, Sabah. Serogroup was determined on the basis of ELISA (enzyme-linked immunosorbent assay). Four different serogroups were identified which were serogroup B, C, D, and E. DNA (deoxyribonucleic acid) was extracted and PCR (polymerase chain reaction) was performed using primers which were designed to amplify the CRISPR array in Salmonella genome. Our results indicate that there is a correlationship between serogroup obtained using ELISA and the profile generated by CRISPR typing. Conclusion, significance and impact of study: CRISPR typing has the potential to be applied for the genotyping of Salmonella.

Related Concepts

DNA
Enzyme-Linked Immunosorbent Assay
Genome
Laboratory
Mycobacterium tuberculosis
Oligonucleotides
Polymerase Chain Reaction
Oligonucleotide Primers
Typing Classification
Salmonella enterica

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