Genotyping of Toxoplasma gondii by multilocus PCR-RFLP markers: a high resolution and simple method for identification of parasites

International Journal for Parasitology
C SuJ P Dubey

Abstract

It was generally believed that Toxoplasma gondii had a clonal population structure with three predominant lineages, namely types I, II and III. This was largely based on genotyping of more than 100 T. gondii isolates originating from a variety of human and animal sources in North America and Europe. Recent genotyping studies on T. gondii strains from wild animals or human patients from different geographical regions revealed the high frequency of non-archetypal genotypes, suggesting the overall diversity of the T. gondii population might be much higher than we thought. However, as most genotyping studies had relied on a few biallelic markers, the resolution and discriminative power of identifying parasite isolates were quite low. To date, there is no commonly used set of markers to genotype T. gondii strains and it is not feasible to compare results from different laboratories. Here, we developed nine PCR-restriction fragment length polymorphism markers with each of them capable of distinguishing the three archetypal T. gondii alleles in one restriction-enzyme reaction by agarose gel electrophoresis. Genotyping 46 T. gondii isolates from different sources using these markers showed that they could readily distinguish the archet...Continue Reading

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Citations

Nov 20, 2012·Parasitology Research·Li YuChristine A Sundermann
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chunlei SuL David Sibley
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·L David SibleyBenjamin M Rosenthal
Apr 2, 2013·Applied and Environmental Microbiology·Hongchao SunQuan Liu
Apr 12, 2012·Clinical Microbiology Reviews·Florence Robert-Gangneux, Marie-Laure Dardé
Oct 1, 2010·Journal of Clinical Microbiology·Daniel AjzenbergMarie-Laure Dardé
Nov 14, 2012·The Journal of Parasitology·Francesca ManciantiAlessandro Poli
Jun 26, 2008·The Journal of Parasitology·J P DubeyC Su
Oct 18, 2008·The Journal of Parasitology·J P DubeyC Su
Jul 28, 2011·The Journal of Parasitology·Vanessa MuradianSolange Maria Gennari
Dec 10, 2009·Future Microbiology·Vera Lucia Pereira-ChioccolaChunlei Su
Jun 4, 2010·The American Journal of Tropical Medicine and Hygiene·Sonia BoughattasAïda Bouratbine
Mar 17, 2015·International Journal of Food Microbiology·C BacciF Brindani
Sep 17, 2014·The Journal of Experimental Medicine·Ji Su MaMasahiro Yamamoto
Jan 24, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·B R VijaykumarR S Jayshree
Mar 20, 2010·Veterinary Parasitology·Alessandra M A RagozoSolange M Gennari
Nov 17, 2015·Animal Health Research Reviews·Heidi M Wyrosdick, John J Schaefer
Mar 10, 2016·The Korean Journal of Parasitology·Leila DanehchinAbolghasem Naghibi
Aug 22, 2009·International Journal for Parasitology·D C HerrmannG Schares
Feb 17, 2009·International Journal for Parasitology·Michael E Grigg, Natarajan Sundar
Sep 23, 2008·Veterinary Parasitology·J P DubeyC Su
Sep 19, 2015·Journal of Comparative Pathology·V HerderP Wohlsein

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