Genotyping of Trypanosoma cruzi DTUs and Trypanosoma rangeli genetic groups in experimentally infected Rhodnius prolixus by PCR-RFLP

Acta Tropica
Amanda R N SáMônica Lúcia Gomes

Abstract

The specific detection and genetic typing of trypanosomes that infect humans, mammalian reservoirs, and vectors is crucial for diagnosis and epidemiology. We utilized a PCR-RFLP assay that targeted subunit II of cytochrome oxidase and 24Sα-rDNA to simultaneously detect and discriminate six Trypanosoma cruzi discrete typing units (DTUs) and two genetic groups of Trypanosoma rangeli (KP1+/KP1-) in intestinal contents of experimentally infected Rhodnius prolixus. The PCR assays showed that in 23 of 29 (79.4%) mixed infections with the six T. cruzi DTUs and mixed infections with individual DTUs and/or groups KP1+ and KP1-, both parasites were successfully detected. In six mixed infections that involved TcIII, the TcI, TcII, TcV, and TcVI DTUs predominated to the detriment of TcIII, indicating the selection of genetic groups. Interactions between different genetic groups and vectors may lead to genetic selection over TcIII. The elimination of this DTU by the immune system of the vector appears unlikely because TcIII was present in other mixed infections (TcIII/TcIV and TcIII/KP1+). Both molecular markers used in this study were sensitive and specific, demonstrating their usefulness in a wide geographical area where distinct genotype...Continue Reading

References

Oct 1, 1992·Molecular and Biochemical Parasitology·A M MacedoS D Pena
May 1, 1995·Journal of Invertebrate Pathology·C B MelloP Azambuja
May 1, 1994·The Journal of Eukaryotic Microbiology·M SteindelA J Romanha
Jan 1, 1994·Molecular and Biochemical Parasitology·R F RecinosJ E Donelson
May 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·J R CouraD A Campbell
Dec 20, 1996·Molecular and Biochemical Parasitology·R P SoutoB Zingales
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·J R CouraO Fernandes
Mar 31, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·O FernandesJ R Coura
Jan 5, 2002·Acta Tropica·Gustavo A VallejoMário Steindel
Aug 26, 2003·Memórias do Instituto Oswaldo Cruz·Felipe Guhl, Gustavo Adolfo Vallejo
Sep 11, 2003·Clinical and Diagnostic Laboratory Immunology·Miguel Angel ChiurilloJosé Luis Ramírez
Mar 27, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·Paula Ximena PaviaConcepción Judith Puerta
Jun 12, 2008·Revista Da Sociedade Brasileira De Medicina Tropical·João Carlos Pinto DiasDalmo Correia
Nov 11, 2008·Acta Tropica·G A VallejoG A Schaub
Dec 10, 2009·The American Journal of Tropical Medicine and Hygiene·Michael D LewisMichael A Miles
Dec 23, 2009·Memórias do Instituto Oswaldo Cruz·B ZingalesUNKNOWN Second Satellite Meeting
Aug 31, 2010·Trends in Parasitology·Eloi S GarciaGünter A Schaub
Oct 30, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·P B HamiltonM M G Teixeira
Jan 10, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Bianca ZingalesNancy R Sturm
May 1, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·C Miguel PintoVeronika M Cottontail
Dec 24, 2013·Parasites & Vectors·Amanda Regina Nichi de SáMônica Lúcia Gomes
Mar 7, 2014·Parasites & Vectors·Catarina A C AraújoAna M Jansen
Jun 12, 2014·Parasites & Vectors·Rosa Amélia Gonçalves SantanaMaria das Graças Vale Barbosa
Sep 19, 2014·PLoS Neglected Tropical Diseases·Patrícia Hermes StocoEdmundo Carlos Grisard

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Citations

Oct 16, 2015·PloS One·Luciana de Lima FerreiraAlessandra Aparecida Guarneri
Feb 21, 2018·PLoS Neglected Tropical Diseases·Glaucia Diniz AlessioOlindo Assis Martins-Filho
Jun 27, 2019·Vector Borne and Zoonotic Diseases·Ricardo Nascimento DrozinoMax Jean de Ornelas Toledo
Nov 22, 2017·Revista Da Sociedade Brasileira De Medicina Tropical·Elaine Schultz DworakMax Jean de Ornelas Toledo

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