Genotyping Toxoplasma gondii with the B1 Gene in Naturally Infected Sheep from an Endemic Region in the Pacific Coast of Mexico

Vector Borne and Zoonotic Diseases
Williams Arony Martinez-FloresPablo Maravilla

Abstract

Toxoplasma gondii is a protozoan parasite with a broad ecological valence, which has been detected in a wide range of hosts and landscapes. Although the genus is considered monospecific, in recent years it has been demonstrated to exhibit more genetic variability than previously known. In Mexico, there are few genotyping studies, which suggest that classical, autochthonous, and atypical strains are circulating. The goal of this study was to describe T. gondii genetic diversity in naturally infected sheep from Colima, Mexico. This is a good site to study ecological aspects of this parasite since it is located between the Nearctic and Neotropical ecozones and it includes domestic and wild risks for transmission. We analyzed 305 tissue samples of semicaptive sheep from six coastal and central zones of Colima and border zones of Michoacán. We used an 803 bp amplicon of the B1 gene to genotype T. gondii and seroprevalence was determined by ELISA. Indexes for genetic diversity and genetic differentiation were calculated and compared with reference strains from North America (NA) and South America (SA). Twenty-three tissue samples were positive for the B1 gene by PCR, which were sequenced. Crude prevalence was 24.4%. The genetic analy...Continue Reading

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Citations

Jan 4, 2021·Acta Parasitologica·Amina BoutellisTahar Kernif
Jan 12, 2021·Journal Für Verbraucherschutz Und Lebensmittelsicherheit = Journal of Consumer Protection and Food Safety·Amin YousefvandShirin Mohammadyari

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Datasets Mentioned

BETA
AF179871.1

Methods Mentioned

BETA
genotyping
ELISA
density gradient centrifugation
PCR

Software Mentioned

BLAST
DnaSP
BLASTN
Figtree
Mesquite
Chromaseq
MrBayes
ClustalW

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