Genus- and species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the 16S/23S intergenic spacer region of the rRNA operon

Canadian Journal of Microbiology
T A GrahamJ E Thomas

Abstract

In this study, the 16S/23S rRNA intergenic spacer (IGS) regions of six Listeria species were examined. DNA bands of 590 and 340 bp were observed following polymerase chain reaction (PCR) amplification of DNA from Listeria monocytogenes, Listeria innocua, Listeria seeligeri, Listeria welshimeri, and Listeria ivanovii strains with generic rRNA IGS oligonucleotide primers. For strains of Listeria grayi subspp. grayi and murrayi, DNA band sizes of 550 and 340 bp were observed with this primer pair. DNA bands of these sizes were not observed for other Gram-negative or- positive bacteria in this PCR assay. Four RsaI digestion profiles were noted for the Listeria PCR products. Listeria monocytogenes strains had one profile; L. innocua strains had a second; L seeligeri, L. welshimeri, and L ivanovii strains had a third; and L. grayi strains had a fourth. The small and large 16S/23S rRNA IGSs of L. monocytogenes ATCC 15313 were identical in the first 58 5' and the last 169 3' nucleotides. However, the large rRNA IGS contained a central 267-bp region with tRNA(Ile) and tRNA(Ala) genes. Large rRNA 16S/23S IGS nucleotide sequence data has not been previously reported. This data was used to develop novel Listeria genus-specific and L.monocy...Continue Reading

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Citations

Apr 17, 2012·Applied and Environmental Microbiology·Brian D SaudersMartin Wiedmann
Jul 4, 2001·Clinical Microbiology Reviews·J A Vázquez-BolandJ Kreft
Nov 19, 2015·Frontiers in Microbiology·Jodi Woan-Fei LawLearn-Han Lee
Oct 13, 2005·FEMS Microbiology Reviews·Uta GasanovPhilip M Hansbro
Dec 12, 2001·Molecular and Cellular Probes·A IngianniR Pompei
Mar 22, 2003·FEMS Immunology and Medical Microbiology·Michael SchmidKarl-Heinz Schleifer
Jun 28, 2003·FEMS Microbiology Letters·Dongyou LiuMark L Lawrence
Jul 24, 2004·Journal of Food Protection·Wei ZhangStephen J Knabel
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Vincenzina Fusco, Grazia Marina Quero
May 1, 1999·Applied and Environmental Microbiology·D M Norton, C A Batt

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