Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses

Applied and Environmental Microbiology
Laura L LeeRobert M Kelly

Abstract

Metagenomic data from Obsidian Pool (Yellowstone National Park, USA) and 13 genome sequences were used to reassess genus-wide biodiversity for the extremely thermophilic Caldicellulosiruptor The updated core genome contains 1,401 ortholog groups (average genome size for 13 species = 2,516 genes). The pangenome, which remains open with a revised total of 3,493 ortholog groups, encodes a variety of multidomain glycoside hydrolases (GHs). These include three cellulases with GH48 domains that are colocated in the glucan degradation locus (GDL) and are specific determinants for microcrystalline cellulose utilization. Three recently sequenced species, Caldicellulosiruptor sp. strain Rt8.B8 (renamed here Caldicellulosiruptor morganii), Thermoanaerobacter cellulolyticus strain NA10 (renamed here Caldicellulosiruptor naganoensis), and Caldicellulosiruptor sp. strain Wai35.B1 (renamed here Caldicellulosiruptor danielii), degraded Avicel and lignocellulose (switchgrass). C. morganii was more efficient than Caldicellulosiruptor bescii in this regard and differed from the other 12 species examined, both based on genome content and organization and in the specific domain features of conserved GHs. Metagenomic analysis of lignocellulose-enric...Continue Reading

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Citations

Nov 6, 2018·Folia Microbiologica·Bhagwan Rekadwad, Juan M Gonzalez
Jul 31, 2019·Extremophiles : Life Under Extreme Conditions·Laura L LeeRobert M Kelly
Aug 9, 2019·Nature Communications·Christopher T StraubRobert M Kelly
Aug 9, 2019·Journal of Industrial Microbiology & Biotechnology·Asma M A M KhanSara E Blumer-Schuette
Dec 15, 2020·International Journal of Biological Macromolecules·Nisha SinghMunish Puri
Mar 16, 2019·Current Opinion in Biotechnology·James R CrosbyRobert M Kelly
Jun 4, 2019·Chemosphere·Rajesh Kumar PrasadDharmendra Kumar Gupta
Mar 9, 2021·Environmental Microbiology Reports·Ryan G BingRobert M Kelly

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