Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina.

Virus Genes
Verónica TruccoFabian Giolitti

Abstract

Alfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has been unknown until now. Here we show that the black aphid Aphis craccivora can transmit AEV-1. The CP sequence identity among 16 AEV-1 isolates from Argentina was from 98 to 100% and from 98.9 to 100% at nucleotide and amino acid levels, respectively, indicating a low level of sequence variation among these isolates. The phylogenetic analysis based on the complete nucleotide sequence of the CP gene indicated that AEV-1 isolates are closely related and clustered in a monophyletic group. These results suggest that AEV-1 has spread very recently in Argentina. In the present study, we report the geographical distribution of AEV-1 in the main alfalfa-growing areas of Argentina and, for the first time, identify an insect vector and describe the CP gene diversity of an enamovirus worldwide.

References

May 6, 2003·Annual Review of Phytopathology·Stewart Gray, Frederick E Gildow
Sep 19, 2012·Methods in Molecular Biology·Domenico Bosco, Rosemarie Tedeschi
Sep 27, 2014·PloS One·Brejnev Muhizi MuhireDarren Patrick Martin
Jun 26, 2015·Journal of Virology·Philippe RoumagnacMichel Peterschmitt
Oct 9, 2016·Plant Science : an International Journal of Experimental Plant Biology·Tiziana Mascia, Donato Gallitelli
May 20, 2017·Annual Review of Phytopathology·Coralie PicardGaël Thébaud
Jul 8, 2017·Genome Announcements·Lev G NemchinovDimitre S Mollov
Dec 7, 2017·Archives of Virology·Nicolás BejermanFabián Giolitti
May 4, 2018·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Jun 1, 2011·Plant Disease·N BejermanS Lenardon
Apr 3, 2019·Virus Research·Nicolás BejermanFabián Giolitti

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

GIS
AmplifX
Primer
MEGA X
SDT
Staden package
DIVA
MUSCLE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Department of State Publication. Background Notes Series
United States. Department of State. Bureau of Public Affairs. Office of Public Communication
Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases
Lancet
R Whitecross
Department of State Publication. Background Notes Series
United States. Department of State. Bureau of Public Affairs
© 2021 Meta ULC. All rights reserved