Geographically widespread honeybee-gut symbiont subgroups show locally distinct antibiotic-resistant patterns

Molecular Ecology
Jane LudvigsenKnut Rudi

Abstract

How long-term antibiotic treatment affects host bacterial associations is still largely unknown. The honeybee-gut microbiota has a simple composition, so we used this gut community to investigate how long-term antibiotic treatment affects host-associated microbiota. We investigated the phylogenetic relatedness, genomic content (GC percentage, genome size, number of genes and CRISPR) and antibiotic-resistant genes (ARG) for strains from two abundant members of the honeybee core gut microbiota (Gilliamella apicola and Snodgrassella alvi). Domesticated honeybees are subjected to geographically different management policies, so we used two research apiaries, representing different antibiotic treatment regimens in their apiculture: low antibiotic usage (Norway) and high antibiotic usage (Arizona, USA). We applied whole-genome shotgun sequencing on 48 G. apicola and 22 S. alvi. We identified three predominating subgroups of G. apicola in honeybees from both Norway and Arizona. For G. apicola, genetic content substantially varied between subgroups and distance similarity calculations showed similarity discrepancy between subgroups. Functional differences between subgroups, such as pectin-degrading enzymes (G. apicola), were also ident...Continue Reading

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Citations

Nov 23, 2017·Molecular Ecology·Kasie RaymannNancy A Moran
Nov 24, 2018·Microbial Ecology·Céline RibièrePaul W O'Toole
Mar 14, 2019·Molecular Ecology·Kirsten M EllegaardPhilipp Engel
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Jan 9, 2018·Current Opinion in Microbiology·Germán Bonilla-Rosso, Philipp Engel
Aug 15, 2021·Nature Reviews. Genetics·Julie Perreau, Nancy A Moran

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