Geometry influences inflammatory host cell response and remodeling in tissue-engineered heart valves in-vivo.

Scientific Reports
Sarah E MottaMaximilian Y Emmert

Abstract

Regenerative tissue-engineered matrix-based heart valves (TEM-based TEHVs) may become an alternative to currently-used bioprostheses for transcatheter valve replacement. We recently identified TEM-based TEHVs-geometry as one key-factor guiding their remodeling towards successful long-term performance or failure. While our first-generation TEHVs, with a simple, non-physiological valve-geometry, failed over time due to leaflet-wall fusion phenomena, our second-generation TEHVs, with a computational modeling-inspired design, showed native-like remodeling resulting in long-term performance. However, a thorough understanding on how TEHV-geometry impacts the underlying host cell response, which in return determines tissue remodeling, is not yet fully understood. To assess that, we here present a comparative samples evaluation derived from our first- and second-generation TEHVs. We performed an in-depth qualitative and quantitative (immuno-)histological analysis focusing on key-players of the inflammatory and remodeling cascades (M1/M2 macrophages, α-SMA+- and endothelial cells). First-generation TEHVs were prone to chronic inflammation, showing a high presence of macrophages and α-SMA+-cells, hinge-area thickening, and delayed endoth...Continue Reading

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Citations

Feb 24, 2021·European Heart Journal·Valery L VisserMaximilian Y Emmert
May 10, 2021·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Sarah E MottaMaximilian Y Emmert
Jun 4, 2021·Acta Biomaterialia·Marcus GroundJillian Cornish
Jul 16, 2021·Frontiers in Cell and Developmental Biology·Eric K N GähwilerMaximilian Y Emmert

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Methods Mentioned

BETA
scanning electron microscopy

Software Mentioned

ARRIVE
ImageJ
Panoramic Viewer
Axio Scan . Z1 ZEN lite
Tinkercad

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