Germ cell development in the Honeybee (Apis mellifera); vasa and nanos expression

BMC Developmental Biology
Peter K Dearden

Abstract

Studies of specification of germ-cells in insect embryos has indicated that in many taxa the germ cells form early in development, and their formation is associated with pole plasm, germ plasm or an organelle called the oosome. None of these morphological features associated with germ cell formation have been identified in the Honeybee Apis mellifera. In this study I report the cloning and expression analysis of Honeybee homologues of vasa and nanos, germ cell markers in insects and other animals. Apis vasa and nanos RNAs are present in early honeybee embryos, but the RNAs clear rapidly, without any cells expressing these germ cell markers past stage 2. These genes are then only expressed in a line of cells in the abdomen from stage 9 onwards. These cells are the developing germ cells that are moved dorsally by dorsal closure and are placed in the genital ridge. This study of the expression of germ cell markers in the honeybee implies that in this species either germ cells are formed by an inductive event, late in embryogenesis, or they are formed early in development in the absence of vasa and nanos expression. This contrasts with germ cell development in other members of the Hymenoptera, Diptera and Lepidoptera.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D L Roussell, K L Bennett
Oct 15, 1996·Genes & Development·A Dahanukar, R P Wharton
Jun 2, 1998·Development Genes and Evolution·M GrbićM R Strand
Feb 27, 1999·Genes & Development·K A LawsonB L Hogan
Oct 29, 1999·Genes & Development·J Sonoda, R P Wharton
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·D H CastrillonC P Crum
Dec 29, 2000·International Review of Cytology·A P Mahowald
Mar 17, 2001·Development Genes and Evolution·H Nakao
Sep 3, 2002·Mechanisms of Development·Katja MüllerErez Raz
Nov 28, 2002·Developmental Biology·Chun-che ChangMichael Akam
Jan 22, 2003·Development Genes and Evolution·Peter DeardenCam Donly
Feb 8, 2003·Current Biology : CB·Sabbi LallNipam H Patel
Apr 12, 2003·Development Genes and Evolution·Jadwiga JaruzelskaRenee A Reijo Pera
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Aug 30, 2003·Science·Masayuki TsudaYumiko Saga
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yayoi ToyookaToshiaki Noce
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·David H MathewsDouglas H Turner
Jul 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·David M DonnellMichael R Strand
Jul 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yoshiki HayashiSatoru Kobayashi
Aug 28, 2004·Evolution & Development·Raquel TorrasSergio González-Crespo
May 17, 2005·Insect Biochemistry and Molecular Biology·Eric CalvoAnthony A James
Jul 19, 2005·Development Genes and Evolution·Peter W Osborne, Peter K Dearden

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Citations

Mar 3, 2007·Development Genes and Evolution·Chun-Che ChangTing-Yu Huang
Jun 11, 2008·Development Genes and Evolution·Taro MitoSumihare Noji
Oct 31, 2006·Nature·UNKNOWN Honeybee Genome Sequencing Consortium
Jan 20, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Abderrahman Khila, Ehab Abouheif
Oct 27, 2006·Genome Research·Peter K DeardenMartin Beye
Oct 12, 2010·Development·Jeremy A Lynch, Claude Desplan
Jun 22, 2012·PLoS Neglected Tropical Diseases·Danielle E SkinnerPaul J Brindley
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Seth DonougheCassandra G Extavour
Apr 23, 2017·Genesis : the Journal of Genetics and Development·A G CridgeP K Dearden
Jul 1, 2009·Genetics and Molecular Biology·Erica Donato Tanaka, Klaus Hartfelder
Apr 19, 2016·Current Opinion in Insect Science·Honghu Quan, Jeremy A Lynch
Feb 6, 2018·Cellular and Molecular Life Sciences : CMLS·Evi De KeuckelaereFrans van Roy
Apr 25, 2012·Development Genes and Evolution·Paula Rezende-TeixeiraGláucia Maria Machado-Santelli
Dec 10, 2015·Zoological Letters·Mercedes Maceren-PatesMichiyasu Yoshikuni
Jan 1, 2012·Wiley Interdisciplinary Reviews. Developmental Biology·Jeremy A LynchSusan J Brown
Nov 10, 2018·Scientific Reports·M OtteM Beye
Feb 13, 2019·BMC Evolutionary Biology·Carrie A Whittle, Cassandra G Extavour
Feb 13, 2008·Gene Expression Patterns : GEP·Guoli ZhaoHongliang Zhou
Nov 4, 2008·Biochimica Et Biophysica Acta·Miriam I RosenbergClaude Desplan
Dec 23, 2009·Developmental Biology·Megan J WilsonPeter K Dearden

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Datasets Mentioned

BETA
DQ288392

Methods Mentioned

BETA
PCR

Software Mentioned

Amvasa
tBlastN
MFOLD
Phylip
MrBayes
gnomon
ENSEMBL
ClustalX

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