Germ Cell Nuclear Factor (GCNF) Represses Oct4 Expression and Globally Modulates Gene Expression in Human Embryonic Stem (hES) Cells.

The Journal of Biological Chemistry
Hongran WangAustin J Cooney

Abstract

Oct4 is considered a key transcription factor for pluripotent stem cell self-renewal. It binds to specific regions within target genes to regulate their expression and is downregulated upon induction of differentiation of pluripotent stem cells; however, the mechanisms that regulate the levels of human Oct4 expression remain poorly understood. Here we show that expression of human Oct4 is directly repressed by germ cell nuclear factor (GCNF), an orphan nuclear receptor, in hES cells. Knockdown of GCNF by siRNA resulted in maintenance of Oct4 expression during RA-induced hES cell differentiation. While overexpression of GCNF promoted repression of Oct4 expression in both undifferentiated and differentiated hES cells. The level of Oct4 repression was dependent on the level of GCNF expression in a dose-dependent manner. mRNA microarray analysis demonstrated that overexpression of GCNF globally regulates gene expression in undifferentiated and differentiated hES cells. Within the group of altered genes, GCNF down-regulated 36% of the genes, and up-regulated 64% in undifferentiated hES cells. In addition, GCNF also showed a regulatory gene pattern that is different from RA treatment during hES cell differentiation. These findings in...Continue Reading

References

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Dec 11, 1996·Biochimica Et Biophysica Acta·U Süsens, U Borgmeyer
May 2, 1997·Biochimica Et Biophysica Acta·M KapelleW D Schleuning
Nov 6, 1998·Science·J A ThomsonJ M Jones
Dec 28, 1999·Reproduction, Fertility, and Development·J NicholsM Buehr
Nov 13, 2001·Developmental Cell·G FuhrmannA J Cooney
Sep 5, 2002·Biochemical and Biophysical Research Communications·Mi-Hyun KimFuyuki Ishikawa
Oct 17, 2002·The Journal of Biological Chemistry·Zi-Jian LanAustin J Cooney
Dec 8, 2004·Best Practice & Research. Clinical Obstetrics & Gynaecology·Yoel Shufaro, Benjamin E Reubinoff
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Apr 25, 2006·Biochemical and Biophysical Research Communications·Noriko SatoKen-ichi Arai
Feb 21, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wado AkamatsuDerek van der Kooy

❮ Previous
Next ❯

Citations

Oct 24, 2017·Journal of Cellular Biochemistry·Sung-Won ParkJae-Hwan Kim
Jun 24, 2017·Cellular and Molecular Life Sciences : CMLS·Dimitrios GkikasPanagiotis K Politis
Sep 22, 2019·Animals : an Open Access Journal From MDPI·Xiya FangRuihua Dang
Jul 19, 2019·Cell Communication and Signaling : CCS·Yinfang WangPeng Zhang
May 26, 2017·Epigenetics & Chromatin·Ruiqi Liao, Craig A Mizzen

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Chip
electrophoresis
Immunoprecipitation
electrophoretic mobility shift assay
PCA

Software Mentioned

DNA Chip Analyzer

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.