Germ cell transplantation and testis tissue xenografting in mice.

Journal of Visualized Experiments : JoVE
Lin TangIna Dobrinski

Abstract

Germ cell transplantation was developed by Dr. Ralph Brinster and colleagues at the University of Pennsylvania in 1994(1,2). These ground-breaking studies showed that microinjection of germ cells from fertile donor mice into the seminiferous tubules of infertile recipient mice results in donor-derived spermatogenesis and sperm production by the recipient animal(2). The use of donor males carrying the bacterial β-galactosidase gene allowed identification of donor-derived spermatogenesis and transmission of the donor haplotype to the offspring by recipient animals(1). Surprisingly, after transplantation into the lumen of the seminiferous tubules, transplanted germ cells were able to move from the luminal compartment to the basement membrane where spermatogonia are located(3). It is generally accepted that only SSCs are able to colonize the niche and re-establish spermatogenesis in the recipient testis. Therefore, germ cell transplantation provides a functional approach to study the stem cell niche in the testis and to characterize putative spermatogonial stem cells. To date, germ cell transplantation is used to elucidate basic stem cell biology, to produce transgenic animals through genetic manipulation of germ cells prior to tra...Continue Reading

References

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Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, M R Avarbock
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Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M NaganoR L Brinster
Aug 16, 2002·Nature·Ali HonaramoozStefan Schlatt
Sep 5, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
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May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi KubotaRalph L Brinster
Aug 2, 2005·Animal Reproduction Science·Ina Dobrinski
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May 22, 2007·Animal Reproduction Science·Lucía ArreguiIna Dobrinski
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Mar 6, 2009·Reproduction, Fertility, and Development·W ZengI Dobrinski
Apr 18, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Jose R Rodriguez-Sosa, Ina Dobrinski
Nov 19, 2010·Biology of Reproduction·Melissa J OatleyJon M Oatley

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