Germ-line-competent embryonic stem cells of the Chinese Kunming mouse strain with long-term self-renewal ability

Cellular Reprogramming
Xinrong PengQijun Qian


Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we show that KM ESCs can be efficiently derived and maintained in chemically defined N2B27 medium with the presence of two small molecules PD0325901 and CHIR99021 (2i medium). These KM ESCs exhibit all features of ESCs, including long-term self-renewal ability, expression of key molecular markers (Oct4, Nanog, and Sox2), the ability to form teratomas, and the capacity to incorporate into the developing embryo and then transmit through the germ line.


Dec 1, 1995·Current Opinion in Cell Biology·G M Keller
Nov 1, 2001·Annual Review of Cell and Developmental Biology·A G Smith
Jan 14, 2003·Nature Biotechnology·Qi-Long YingAustin Smith
May 19, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuya OgawaHitoshi Niwa
Sep 21, 2004·Oncogene·Ian Chambers, Austin Smith
Jan 12, 2007·Development·Hitoshi Niwa
Dec 22, 2007·Nature·Ian ChambersAustin Smith
Feb 26, 2008·Cell·Jose Silva, Austin Smith
May 24, 2008·Nature·Qi-Long YingAustin Smith
Jun 6, 2009·Nature Medicine·Jennifer NicholsAnne Cooke
May 1, 2012·Cell·Hendrik MarksHendrik G Stunnenberg


Feb 28, 2014·International Journal of Molecular Sciences·Shumin YuZhihua Ren
Sep 14, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Dong AnSheng-Ming Yin
Jan 17, 2015·In Vitro Cellular & Developmental Biology. Animal·Xiaokun LiuBaohua Ma

Methods Mentioned

Flow cytometry

Related Concepts

Chir 99021
Nanog protein, mouse
Pou5f1 protein, mouse
PD 0325901
Sox2 protein, mouse
Cell Differentiation Process

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