Germ-line-competent embryonic stem cells of the Chinese Kunming mouse strain with long-term self-renewal ability

Cellular Reprogramming
Xinrong PengQijun Qian

Abstract

Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we show that KM ESCs can be efficiently derived and maintained in chemically defined N2B27 medium with the presence of two small molecules PD0325901 and CHIR99021 (2i medium). These KM ESCs exhibit all features of ESCs, including long-term self-renewal ability, expression of key molecular markers (Oct4, Nanog, and Sox2), the ability to form teratomas, and the capacity to incorporate into the developing embryo and then transmit through the germ line.

References

Dec 1, 1995·Current Opinion in Cell Biology·G M Keller
Nov 1, 2001·Annual Review of Cell and Developmental Biology·A G Smith
Jan 14, 2003·Nature Biotechnology·Qi-Long YingAustin Smith
May 19, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuya OgawaHitoshi Niwa
Sep 21, 2004·Oncogene·Ian Chambers, Austin Smith
Jan 12, 2007·Development·Hitoshi Niwa
Dec 22, 2007·Nature·Ian ChambersAustin Smith
Feb 26, 2008·Cell·Jose Silva, Austin Smith
May 24, 2008·Nature·Qi-Long YingAustin Smith
Jun 6, 2009·Nature Medicine·Jennifer NicholsAnne Cooke
May 1, 2012·Cell·Hendrik MarksHendrik G Stunnenberg

Citations

Feb 28, 2014·International Journal of Molecular Sciences·Shumin YuZhihua Ren
Sep 14, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Dong AnSheng-Ming Yin
Jan 17, 2015·In Vitro Cellular & Developmental Biology. Animal·Xiaokun LiuBaohua Ma

Methods Mentioned

BETA
Flow cytometry

Related Concepts

Chir 99021
Nanog protein, mouse
Pou5f1 protein, mouse
PD 0325901
Sox2 protein, mouse
Metazoa
Benzamides
Cell Differentiation Process
Diphenylamine
Gametes

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.