Germ Line Deletion Reveals a Nonessential Role of Atypical Mitogen-Activated Protein Kinase 6/Extracellular Signal-Regulated Kinase 3

Molecular and Cellular Biology
Natalia RonkinaMatthias Gaestel

Abstract

Mitogen-activated protein kinase 6/extracellular signal-regulated kinase 3 (MAPK6/ERK3) is an atypical member of the MAPKs. An essential role has been suggested by the perinatal lethal phenotype of ERK3 knockout mice carrying a lacZ insertion in exon 2 due to pulmonary dysfunction and by defects in function, activation, and positive selection of T cells. To study the role of ERK3 in vivo, we generated mice carrying a conditional Erk3 allele with exon 3 flanked by loxP sites. Loss of ERK3 protein was validated after deletion of Erk3 in the female germ line using zona pellucida 3 (Zp3)-cre and a clear reduction of the protein kinase MK5 is detected, providing the first evidence for the existence of the ERK3/MK5 signaling complex in vivo In contrast to the previously reported Erk3 knockout phenotype, these mice are viable and fertile and do not display pulmonary hypoplasia, acute respiratory failure, abnormal T-cell development, reduction of thymocyte numbers, or altered T-cell selection. Hence, ERK3 is dispensable for pulmonary and T-cell functions. The perinatal lethality and lung and T-cell defects of the previous ERK3 knockout mice are likely due to ERK3-unrelated effects of the inserted lacZ-neomycin resistance cassette. The ...Continue Reading

References

Jun 15, 1992·FEBS Letters·F A GonzalezR J Davis
Dec 1, 1994·Molecular and Cellular Biology·A X ZhuJ S Flier
Oct 16, 2003·Molecular and Cellular Biology·Yu ShiMatthias Gaestel
Nov 13, 2004·The EMBO Journal·Stefanie SchumacherMatthias Gaestel
Sep 15, 2006·The Journal of Biological Chemistry·Espen AbergOle-Morten Seternes
Sep 16, 2006·The Journal of Biological Chemistry·Shashi KantMatthias Gaestel
May 29, 2009·The Journal of Biological Chemistry·Espen AbergOle-Morten Seternes
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sonia KlingerSylvain Meloche
Dec 4, 2009·PloS One·Yingjie WuShoshana Yakar
Feb 15, 2011·The Journal of Biological Chemistry·Alina De la Mota-PeynadoAlexander Beeser
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Marie Cargnello, Philippe P Roux
Apr 17, 2012·The Journal of Clinical Investigation·Weiwen LongBert W O'Malley
Jan 30, 2014·PloS One·Miriam MarquisNathalie Labrecque
Mar 4, 2014·Journal of Cellular Physiology·Wei WangWeiwen Long
Dec 19, 2014·Immunology·Julien SiroisNathalie Labrecque

Citations

Apr 22, 2020·ELife·Katarzyna BoguckaKrishnaraj Rajalingam
Oct 19, 2020·Cancer Gene Therapy·Katarzyna BoguckaKrishnaraj Rajalingam

Related Concepts

Exons
Gene Deletion
Germ Cells
LacZ Genes
Lung
Lung Diseases
Lung Neoplasms
Laboratory mice
Phosphotransferases
Protein KINASE

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