Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector

Nature
E RobertsonM Evans

Abstract

Embryonic stem cells isolated directly from mouse embryos can be cultured for long periods in vitro and subsequently repopulate the germ line in chimaeric mice. During the culture period these embryonic cells are accessible for experimental genetic manipulation. Here we report the use of retroviral vectors to introduce exogenous DNA sequences into a stem-cell line and show that these modified cells contribute extensively to the somatic and germ-cell lineages in chimaeric mice. Compared with current methods for manipulation of the mouse genome, this approach has the advantage that powerful somatic-cell genetic techniques can be used to modify and to select cells with germ-line potential, allowing the derivation of transgenic strains with pre-determined genetic changes. We have by this means inserted many proviral vector sequences that provide new chromosomal molecular markers for linkage studies in the mouse and that also may cause insertional mutations.

References

Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D HuszarA Bernstein
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·R H Lovell-BadgeK S Cheah
Jun 1, 1985·Cell·R D Palmiter, R L Brinster
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C StockingW Ostertag
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H van der PuttenI M Verma
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W ReikR Jaenisch
Jul 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·W S RobinsonH Rubin
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K HarbersR Jaenisch

❮ Previous
Next ❯

Citations

Jun 17, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M B CarltonM J Evans
Nov 26, 1999·Journal of Cellular Biochemistry·H SauerM Wartenberg
May 1, 1997·Molecular Reproduction and Development·S Y VatolinO L Serov
Jul 17, 1998·Developmental Genetics·M J SollowayE J Robertson
Jul 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P M IannacconeM R Kuehn
Oct 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Huxley, A Gnirke
Dec 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A L Joyner
Aug 14, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P CarrollS Alonso
Dec 1, 1990·Molecular Reproduction and Development·L PicardK J Betteridge
Dec 1, 1991·Molecular Reproduction and Development·C L BrazolotA M Verrinder Gibbins
Dec 1, 1992·Molecular Reproduction and Development·M A SukoyanS I Bayborodin
Mar 1, 1995·Molecular Reproduction and Development·N OuhibiN J Clarke
Dec 1, 1995·Molecular Reproduction and Development·H KagamiR J Etches
Jan 1, 1992·Cell Biology and Toxicology·P CollodiD W Barnes
Jul 1, 1990·Somatic Cell and Molecular Genetics·M I MunirC T Caskey
Mar 1, 1996·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·A AguzziJ P Steinbach
Sep 15, 1991·Experientia·S Rusconi
Sep 15, 1991·Experientia·L M Houdebine, D Chourrout
Feb 3, 2007·Journal of Applied Genetics·Eduardo O MeloRodolfo Rumpf
Jan 24, 2012·Bulletin of Experimental Biology and Medicine·E V Grigor'evaS M Zakian
Nov 13, 2008·Cytotechnology·Paola RebuzziniSilvia Garagna
Aug 10, 2012·Transgenic Research·Masumi HirabayashiShinichi Hochi
May 1, 1988·Developmental Biology·T DoetschmanN Maeda
Nov 1, 1992·Developmental Biology·Y G MaA Rizzino
Apr 12, 1995·Journal of Immunological Methods·L A Galli-TaliadorosH Körner
Jun 1, 1994·Mutation Research·A SandsA Bradley
Aug 1, 1990·Trends in Biotechnology·T T Chen, D A Powers
Oct 1, 1988·Trends in Genetics : TIG·I J Jackson
Mar 1, 1989·Trends in Genetics : TIG·M R Capecchi
Aug 1, 1989·Trends in Genetics : TIG·J Rossant, A L Joyner
Oct 1, 1992·Trends in Genetics : TIG·R Beddington
Nov 15, 1988·Biochimica Et Biophysica Acta·S J CompereR Jaenisch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.