Germ-plasm specification and germline development in the parthenogenetic pea aphid Acyrthosiphon pisum: Vasa and Nanos as markers

The International Journal of Developmental Biology
Chun-che ChangTschining Chang

Abstract

The germarium, oocytes and embryos of the parthenogenetic viviparous pea aphid Acyrthosiphon pisum are contained within a single ovariole. This species provides an excellent model for studying how maternally-inherited germ plasm is specified and how it is transferred to primordial germ cells. Previous studies have shown that germ cells are first segregated at the embryonic posterior after formation of the blastoderm. We used two cross-reacting antibodies against the conserved germline markers Vasa and Nanos, which specifically identified these presumptive germ cells, to investigate whether germ cells were determined during early development. We observed randomly-distributed weak expression of Vasa signals in the developing oocyte but no localization in the oocyte segregated from the germarium. Localized Vasa was not apparent until it was detected at the posterior in the embryo undergoing the second nuclear division. Nanos, on the other hand, was localized to a nuage-like structure surrounding the nucleus in the developing and segregated oocytes. At the beginning of the oocyte maturation division, Nanos localization shifted to the posterior and could be identified in successive stages until it was incorporated into the germ cell...Continue Reading

Citations

Mar 3, 2007·Development Genes and Evolution·Chun-Che ChangTing-Yu Huang
Jul 20, 2011·Cold Spring Harbor Perspectives in Biology·Ekaterina VoroninaIppei Nagamori
Oct 12, 2010·Development·Jeremy A Lynch, Claude Desplan
Mar 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yuying WangPhillip A Newmark
Apr 19, 2016·Current Opinion in Insect Science·Honghu Quan, Jeremy A Lynch
May 16, 2008·Archives of Insect Biochemistry and Physiology·Chun-che ChangWen-Chang Chang
Jul 17, 2008·Biology of the Cell·Gaël Le TrionnaireDenis Tagu
Feb 27, 2013·Developmental Biology·Elizabeth J DuncanPeter K Dearden
Nov 30, 2006·Cell·Geraldine Seydoux, Robert E Braun
Feb 19, 2013·Developmental Biology·Elizabeth J DuncanPeter K Dearden
Jun 17, 2014·Developmental Biology·Jack E Green, Michael Akam
Jun 9, 2009·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Jin-Xia PengJian-Fang Gui
Jan 25, 2021·Insect Biochemistry and Molecular Biology·Woo-Ram ParkDon-Kyu Kim
Aug 28, 2021·Journal of Molecular Biology·Jan S FasslerBryan T Phillips

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