PMID: 9190262Mar 1, 1997Paper

Germination of Nosema algerae (Microspora) spores: conditional inhibition by D2O, ethanol and Hg2+ suggests dependence of water influxupon membrane hydration and specific transmembrane pathways

The Journal of Eukaryotic Microbiology
E FrixioneA H Undeen

Abstract

The germination of microsporidian spores under conditions expected to affect water flow across the plasma membrane-wall complex was studied by assessing their responses to in vitro stimulation with Na+ or K+. Partial or full substitution of common water with D2O, which more effectively coats ions and electrostatically-charged cell surfaces with relatively stable hydration layers, delayed and inhibited spore germination in a concentration-dependent manner; yet, preincubation in 100% D2O did not change the normal response to standard stimulation. Water structure-breaking conditions, such as an increase in temperature (within the 15 degrees C to 40 degrees C range) or in ionic strength (1- to 10-fold normal), opposed the inhibition by D2O and allowed significant stimulation by Li+, the monovalent cation with the largest hydration diameter and a usually weak stimulant action on the spores. Ethanol, known to reduce water permeation across cell membranes and phospholipid bilayers, also caused a powerful and dose-dependent (1% to 4% v/v) inhibition of spore germination, but pretreatment with ethanol did not affect the normal response. HgCl2, an inhibitor of specific water channels, blocked spore germination at just 250 microM in the n...Continue Reading

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Citations

Feb 13, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C P Vivarès, G Méténier
Dec 29, 2000·Bioelectrochemistry·G LahajnarI Zupancic
May 23, 2001·Microbes and Infection·E Bigliardi, L Sacchi
Oct 2, 2002·Current Opinion in Microbiology·Christian P VivarèsGuy Méténier
Dec 17, 2008·Parasitology·G J Leitch, C Ceballos
Aug 31, 2013·PLoS Genetics·Scott E CampbellBryony A P Williams
Jul 27, 2002·Annual Review of Microbiology·Patrick J Keeling, Naomi M Fast
Jul 12, 2005·International Journal for Parasitology·Yanji Xu, Louis M Weiss
Aug 14, 2009·Cellular Microbiology·Bryony A P Williams
Jul 22, 2005·Journal of Evolutionary Biology·D B Vizoso, D Ebert
May 9, 2006·The Journal of Eukaryotic Microbiology·Verena-Ulrike Bläske-LietzeDrion G Boucias
Feb 26, 2011·Immunological Reviews·L David Sibley
Jul 25, 2020·International Journal for Parasitology·Benjamin AmericusStephen D Atkinson
Sep 26, 2017·Microbiology Spectrum·Bing Han, Louis M Weiss
May 10, 2011·International Journal of Medical Microbiology : IJMM·Wolfgang BohneUwe Gross

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