Jul 2, 2020

Germline Mutations in DNA Repair Genes in Patients With Metastatic Castration-resistant Prostate Cancer

In Vivo
Klaudia HoleckovaMaria Skerenova

Abstract

The aim of this study was to analyse the genetic profiles of metastatic castration-resistant prostate cancer (mCRPC) by using next generation sequencing to identify variants with pathogenic potential in nine DNA repair genes - BRCA1, BRCA2, RAD50, RAD51, RAD51C/D, ATM and ATR. Isolated genomic DNA from peripheral blood of 50 patients with mCRPC was used for the sequencing of 111 genes associated with hereditary cancer on an Illumina platform. Identified variants were tested in Integrative Genomic Viewer, their clinical significance confirmed in databases and their potential impact on protein function predicted by in silico tools. From nine analysed DNA repair genes, we identified 14 relevant variants; three pathogenic variants - BRCA2 (rs80359306), RAD50 (rs786201531) and ATM (rs1555099760), and eleven other variants with pathogenic potential. The pathogenic variants identified in this study are located in evolutionarily conserved regions of proteins and are highly likely to affect DNA repair efficiency.

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Mentioned in this Paper

Massively-Parallel Sequencing
DNA Repair
Rad51 Recombinase
ATM
Genes
Conserved Sequence
Molecular Genetic Technique
RAD50 gene
Comparative Genomic Analysis
RAD51

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