Germline P granules are liquid droplets that localize by controlled dissolution/condensation

Science
Clifford P BrangwynneAnthony A Hyman

Abstract

In sexually reproducing organisms, embryos specify germ cells, which ultimately generate sperm and eggs. In Caenorhabditis elegans, the first germ cell is established when RNA and protein-rich P granules localize to the posterior of the one-cell embryo. Localization of P granules and their physical nature remain poorly understood. Here we show that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which we used to estimate their viscosity and surface tension. As with other liquids, P granules rapidly dissolved and condensed. Localization occurred by a biased increase in P granule condensation at the posterior. This process reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell. Such phase transitions may represent a fundamental physicochemical mechanism for structuring the cytoplasm.

References

Jun 1, 1993·The Journal of Cell Biology·S N Hird, J G White
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·M E GruidlK L Bennett
Nov 1, 2003·Current Biology : CB·Caroline A Spike, Susan Strome
Nov 18, 2003·Annual Review of Genetics·Stephan Q Schneider, Bruce Bowerman
May 8, 2004·Science·Dirk G A L AartsHenk N W Lekkerkerker
Mar 8, 2006·The Journal of Cell Biology·Paul Anderson, Nancy Kedersha
Nov 30, 2006·Cell·Geraldine Seydoux, Robert E Braun
Dec 19, 2006·Current Opinion in Cell Biology·Karen E KaszaDavid A Weitz
Apr 21, 2007·Science·Susan Strome, Ruth Lehmann
May 3, 2007·Nature Reviews. Molecular Cell Biology·Bradley M LundeGabriele Varani

❮ Previous
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Citations

Nov 9, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Zehra F Nizami, Joseph G Gall
Mar 15, 2013·Trends in Cell Biology·Gregory A Newby, Susan Lindquist
Sep 14, 2013·Trends in Biochemical Sciences·Nancy KedershaPaul Anderson
Nov 28, 2012·Nature Cell Biology·Timothy T WeilIlan Davis
Sep 3, 2013·Nature Cell Biology·Marina Feric, Clifford P Brangwynne
Oct 24, 2012·Nature Chemistry·Christopher A StrulsonPhilip C Bevilacqua
Feb 19, 2013·Nature Reviews. Genetics·Ueli GrossniklausSusan Lindquist
Apr 19, 2013·Nature Reviews. Molecular Cell Biology·Carsten Hoege, Anthony A Hyman
Apr 2, 2010·Nature Structural & Molecular Biology·Magdalena StrzeleckaKarla M Neugebauer
Apr 7, 2010·Nature Structural & Molecular Biology·Je-Hyun Yoon, Roy Parker
Feb 6, 2013·Lab on a Chip·Jonathan B BoreykoC Patrick Collier
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Clifford P BrangwynneAnthony A Hyman
Dec 26, 2013·The Journal of Cell Biology·Clifford P Brangwynne
Jul 20, 2011·Cold Spring Harbor Perspectives in Biology·Ekaterina VoroninaIppei Nagamori
Dec 20, 2013·Genes & Development·Stefanie Jonas, Elisa Izaurralde
Jun 27, 2009·Science·Loïc Le Goff, Thomas Lecuit
Oct 25, 2011·Science·Nathan W GoehringStephan W Grill
Sep 1, 2012·Science·Anthony A Hyman, Kai Simons
Jun 18, 2014·PLoS Biology·Miguel CoelhoIva M Tolić
Oct 30, 2013·Cellular and Molecular Life Sciences : CMLS·Mostafa BakhtiMikael Simons
Jan 25, 2014·Science·Hye Yoon ParkRobert H Singer
Oct 26, 2013·Trends in Cell Biology·Fabrizio d'Adda di Fagagna
Jun 2, 2014·The Journal of Chemical Physics·William M JacobsDaan Frenkel
Jan 24, 2014·Biomicrofluidics·Yang SongHo Cheung Shum
Apr 10, 2014·Accounts of Chemical Research·Saikat ChakrabortyYamuna Krishnan
Jul 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ritsuya NiwayamaAkatsuki Kimura
Sep 3, 2014·The Journal of Cell Biology·Jeffrey A Toretsky, Peter E Wright
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Anthony A HymanFrank Jülicher
Sep 30, 2014·Nature Biotechnology·Michele CastellanaNed S Wingreen
Jun 22, 2014·Current Opinion in Chemical Biology·Mei LiStephen Mann
Feb 5, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Erica A FrankelChristine D Keating
Dec 17, 2014·International Journal of Molecular Sciences·Irina M KuznetsovaVladimir N Uversky
Feb 26, 2015·The Journal of Cell Biology·ChangHwan LeeAmy S Gladfelter
Feb 9, 2012·The Journal of Cell Biology·Jimmy Ouellet, Yves Barral
Apr 27, 2011·The Journal of Cell Biology·Nathan W GoehringAnthony A Hyman
May 1, 2013·The Journal of Cell Biology·Yun R LiAaron D Gitler
Mar 16, 2011·The Journal of Cell Biology·Dustin L UpdikeSusan Strome
Mar 16, 2011·The Journal of Cell Biology·Momoyo HanazawaAsako Sugimoto
Jan 6, 2016·Cell Communication and Signaling : CCS·Diana M Mitrea, Richard W Kriwacki

❮ Previous
Next ❯

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